Journal article

Authors list: Ringseis, Robert; Wächter, Sophia; Cohrs, Imke; Eder, Klaus; Grünberg, Walter

Publication year: 2024

Pages: 5178-5189

Journal: Journal of Dairy Science

Volume number: 107

Issue number: 7

ISSN: 0022-0302

eISSN: 1525-3198

Open access status: Gold

DOI Link: https://doi.org/10.3168/jds.2023-24099

Publisher: Elsevier

Abstract: 
Although dietary phosphorus (P) deprivation extending from the dry period into early lactation impairs health and productivity of cows, restricting dietary P supply during the dry period not only appears to be innocuous but rather effectively mitigates hypocalcemia during the first wk of lactation. To investigate possible negative metabolic effects of P deprivation during the dry period, the present study tested the hypothesis that restricted dietary P supply during the dry period alters the liver transcriptome of dairy cows during the periparturient period. Thirty late-pregnant multiparous Holstein-Friesian dairy cows entering their second, third, or fourth lactation were assigned to either a dry cow ration with low (LP, 0.16% P in DM) or adequate P content (AP, 0.35% in DM) during the last 4 wk of the dry period (n = 15/group). Liver transcriptomics, which was carried out in a subset of 5 second-parity cows of each group (n = 5), and determination of selected hormones and metabolites in blood of all cows, was performed similar to 1 wk before calving and on d 3 postpartum. Liver tissue specimens and blood samples were obtained by a micro-invasive biopsy technique from the right tenth intercostal space and puncture of a jugular vein, respectively. One hundred seventy-five hepatic transcripts were expressed differentially between LP versus AP cows in late pregnancy, and 165 transcripts differed between LP versus AP cows in early lactation (fold change >1.3 and <-1.3, P < 0.05). In late pregnancy, the enriched biological processes of the upregulated and the downregulated transcripts were mainly related to immune processes and signal transduction (P < 0.05), respectively. In early lactation, the enriched biological processes of the upregulated and the downregulated transcripts were involved in mineral transport and biotransformation (P < 0.05), respectively. The plasma concentrations of the hormones and acute-phase proteins (progesterone, insulin-like growth factor 1, serum amyloid alpha, haptoglobin, and 17 beta-estradiol) determined were not affected by P supply. These results suggest that P deprivation during the dry period moderately affects the liver transcriptome of cows in late pregnancy and early lactation, and causes no effects on important plasma hormones and acute-phase proteins indicating no obvious impairment of health or metabolism of the cows.

Harvard Citation style: Ringseis, R., Wächter, S., Cohrs, I., Eder, K. and Grünberg, W. (2024) Effect of dietary phosphorus deprivation during the dry period on the liver transcriptome of high-yielding periparturient dairy cows, Journal of Dairy Science, 107(7), pp. 5178-5189. https://doi.org/10.3168/jds.2023-24099

APA Citation style: Ringseis, R., Wächter, S., Cohrs, I., Eder, K., & Grünberg, W. (2024). Effect of dietary phosphorus deprivation during the dry period on the liver transcriptome of high-yielding periparturient dairy cows. Journal of Dairy Science. 107(7), 5178-5189. https://doi.org/10.3168/jds.2023-24099