Journal article

Authors list: Wen, Gaiping; Eder, Klaus; Ringseis, Robert

Publication year: 2021

Journal: International Journal of Molecular Sciences

Volume number: 22

Issue number: 9

eISSN: 1422-0067

Open access status: Gold

DOI Link: https://doi.org/10.3390/ijms22094373

Publisher: MDPI

Abstract: 

Recently, ER stress induced by tunicamycin (TM) was reported to inhibit the expression of key genes involved in thyroid hormone synthesis, such as sodium/iodide symporter (NIS), thyroid peroxidase (TPO) and thyroglobulin (TG), and their regulators such as thyrotropin receptor (TSHR), thyroid transcription factor-1 (TTF-1), thyroid transcription factor-2 (TTF-2) and paired box gene 8 (PAX-8), in FRTL-5 thyrocytes. The present study tested the hypothesis that resveratrol (RSV) alleviates this effect of TM in FRTL-5 cells. While treatment of FRTL-5 cells with TM alone (0.1 mu g/mL) for 48 h strongly induced the ER stress-sensitive genes heat shock protein family A member 5 (HSPA5) and DNA damage inducible transcript 3 (DDIT3) and repressed NIS, TPO, TG, TSHR, TTF-1, TTF-2 and PAX-8, combined treatment with TM (0.1 mu g/mL) and RSV (10 mu M) for 48 h attenuated this effect of TM. In conclusion, RSV alleviates TM-induced ER stress and attenuates the strong impairment of expression of genes involved in thyroid hormone synthesis and their regulators in FRTL-5 thyrocytes exposed to TM-induced ER stress. Thus, RSV may be useful for the treatment of specific thyroid disorders, provided that strategies with improved oral bioavailability of RSV are applied.

Harvard Citation style: Wen, G., Eder, K. and Ringseis, R. (2021) Resveratrol Alleviates the Inhibitory Effect of Tunicamycin-Induced Endoplasmic Reticulum Stress on Expression of Genes Involved in Thyroid Hormone Synthesis in FRTL-5 Thyrocytes, International Journal of Molecular Sciences, 22(9), Article 4373. https://doi.org/10.3390/ijms22094373

APA Citation style: Wen, G., Eder, K., & Ringseis, R. (2021). Resveratrol Alleviates the Inhibitory Effect of Tunicamycin-Induced Endoplasmic Reticulum Stress on Expression of Genes Involved in Thyroid Hormone Synthesis in FRTL-5 Thyrocytes. International Journal of Molecular Sciences. 22(9), Article 4373. https://doi.org/10.3390/ijms22094373