Journal article

Authors list: Zorn, H; Peters, T; Nimtz, M; Berger, RG

Publication year: 2005

Pages: 4832-4838

Journal: Proteomics: Proteomics and Systems Biology

Volume number: 5

Issue number: 18

ISSN: 1615-9853

DOI Link: https://doi.org/10.1002/pmic.200500015

Publisher: Wiley

Abstract: 
Due to their unique capability to attack lignified biopolymers, extracellular enzymes of white-rot fungi enjoy an increasing interest in various fields of white biotechnology. The edible fungus Pleurotus sapidus was selected as a model organism for the analysis of the secretome by means of 2-DE. For enzyme production, the fungus was grown in submerged cultures either on peanut shells or on glass wool as a carrier material. Identification of the secreted enzymes was performed by tryptic digestion, ESI-MS/MS ab initio sequencing, and homology searches against public databases. The spectrum of secreted enzymes comprised various types of hydrolases and lignolytic enzymes of the manganese peroxidase/versatile peroxidase family. While peptidases were secreted mainly by the cultures grown on peanut shells, versatile peroxidase type enzymes dominated in the cultures grown on glass wool.

Harvard Citation style: Zorn, H., Peters, T., Nimtz, M. and Berger, R. (2005) The secretome of Pleurotus sapidus, Proteomics: Proteomics and Systems Biology, 5(18), pp. 4832-4838. https://doi.org/10.1002/pmic.200500015

APA Citation style: Zorn, H., Peters, T., Nimtz, M., & Berger, R. (2005). The secretome of Pleurotus sapidus. Proteomics: Proteomics and Systems Biology. 5(18), 4832-4838. https://doi.org/10.1002/pmic.200500015