Journal article

Authors list: Taskova, RM; Zorn, H; Krings, U; Bouws, H; Berger, RG

Publication year: 2006

Pages: 347-350

Journal: A Journal of Biosciences

Volume number: 61

Issue number: 5/6

ISSN: 0939-5075

DOI Link: https://doi.org/10.1515/znc-2006-5-608

Publisher: De Gruyter

Abstract: 
Different techniques were compared for their effectiveness in the disruption of the rigid cell walls of Basidiomycetes. Grinding under liquid nitrogen, stirred glass bead milling and enzymatic cell lysis were applied to the mycelia of Pleurotus sapidus and Lepista irina grown submerged. Each of the disruption procedures was evaluated by testing the quantity and quality of released intracellular metabolites: DNA, RNA, enzymes, and secondary metabolites. The most suitable method for nucleic acid isolation was grinding under liquid nitrogen, while bead mill homogenization was the superior technique for isolation of active enzymes. A new effective method is proposed for isolation of secondary metabolites with the aid of bead milling of fungal mycelia.

Harvard Citation style: Taskova, R., Zorn, H., Krings, U., Bouws, H. and Berger, R. (2006) A comparison of cell wall disruption techniques for the isolation of intracellular metabolites from Pleurotus and Lepista sp., Zeitschrift fur Naturforschung C, 61(5/6), pp. 347-350. https://doi.org/10.1515/znc-2006-5-608

APA Citation style: Taskova, R., Zorn, H., Krings, U., Bouws, H., & Berger, R. (2006). A comparison of cell wall disruption techniques for the isolation of intracellular metabolites from Pleurotus and Lepista sp.. Zeitschrift fur Naturforschung C. 61(5/6), 347-350. https://doi.org/10.1515/znc-2006-5-608