Contribution in an anthology

Authors list: Johansson, H; Zeidler, M

Appeared in: Environmental Responses in Plants: Methods and Protocols

Editor list: Duque, P

Publication year: 2016

Pages: 29-35

ISSN: 1064-3745

ISBN: 978-1-4939-3354-9

eISBN: 978-1-4939-3356-3

DOI Link: https://doi.org/10.1007/978-1-4939-3356-3_4

Title of series: Methods in Molecular Biology

Number in series: 1398

Abstract: 

In response to low or high intensities of light, the chloroplasts in the mesophyll cells of the leaf are able to increase or decrease their exposure to light by accumulating at the upper and lower sides or along the side walls of the cell respectively. This movement, regulated by the phototropin blue light photoreceptors phot1 and phot2, results in a decreased or increased transmission of light through the leaf. This way the plant is able to optimize harvesting of the incoming light or avoid damage caused by excess light. Here we describe a method that indirectly measures the movement of chloroplasts by taking advantage of the resulting change in leaf transmittance. By using a microplate reader, quantitative measurements of chloroplast accumulation or avoidance can be monitored over time, for multiple samples with relatively little hands-on time.

Harvard Citation style: Johansson, H. and Zeidler, M. (2016) Automatic Chloroplast Movement Analysis, in Duque, P. (ed.) Environmental Responses in Plants: Methods and Protocols. New York: Springer - Humana Press, pp. 29-35. https://doi.org/10.1007/978-1-4939-3356-3_4

APA Citation style: Johansson, H., & Zeidler, M. (2016). Automatic Chloroplast Movement Analysis. In Duque, P. (Ed.), Environmental Responses in Plants: Methods and Protocols (pp. 29-35). Springer - Humana Press. https://doi.org/10.1007/978-1-4939-3356-3_4