Journal article

Authors list: Eisenberg, T; Glaeser, SP; Ewers, C; Semmler, T; Nicklas, W; Rau, J; Mauder, N; Hofmann, N; Imaoka, K; Kimura, M; Kämpfer, P

Publication year: 2015

Pages: 4823-4829

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 65

Issue number: 12

ISSN: 1466-5026

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.000654

Publisher: Microbiology Society

Abstract: 
A pleomorphic, Gram-negative, rod-shaped, indole-, oxidase- and catalase-negative, non-spore-forming, non-motile bacterium was isolated in 1979 from the heart of a spinifex hopping mouse (Notomys alexis Thomas, 1922) with septicaemia and stored as Streptobacillus moniliformis in the strain collection of the Animal Health Laboratory, South Perth, Western Australia (AHL 370-1), as well as under CCUG 12425. On the basis of 16SrRNA gene sequence analyses, the strain was assigned to the genus Streptobacillus, with 99.4 % sequence similarity to the type strain of Streptobacillus moniliformis, 95.6 %sequence similarity to the type strain of Streptobacillus hongkongensis and 99.0 %sequence similarity to the type strain of Streptobacillus felis. The clear differentiation of strain AHL 370-1T from Streptobacillus moniliformis, Streptobacillus hongkongensis and Streptobacillus felis was also supported by rpoB, groEL and recA nucleotide and amino acid sequence analysis. Average nucleotide identity was 87.16 % between strain AHL 370-1T and Streptobacillus moniliformis DSM 12112T. Physiological data confirmed the allocation of strain AHL 370-1T to the family Leptotrichiaceae, considering the very similar profiles of enzyme activities and fatty acids compared to closely related species. Within the genus Streptobacillus,isolate AHL 370-1T could also be separated unambiguously from the type strains of Streptobacillus moniliformis, Streptobacillus hongkongensis and Streptobacillus felis by MALDI-TOF mass spectrometry. Two further strains (KWG2 and KWG24) isolated from asymptomatic black rats in Japan were highly similar to AHL 370-1T. On the basis of these data, we propose the novel species Streptobacillus notomytis sp. nov., with the type strain AHL370-1T (=CCUG 12425T=DSM 100026T=CCM 8593T=EF 12425T).

Harvard Citation style: Eisenberg, T., Glaeser, S., Ewers, C., Semmler, T., Nicklas, W., Rau, J., et al. (2015) Streptobacillus notomytis sp. nov., isolated from a spinifex hopping mouse (Notomys alexis Thomas, 1922), and emended description of Streptobacillus Levaditi et al. 1925, Eisenberg et al. 2015 emend, International Journal of Systematic and Evolutionary Microbiology, 65(12), pp. 4823-4829. https://doi.org/10.1099/ijsem.0.000654

APA Citation style: Eisenberg, T., Glaeser, S., Ewers, C., Semmler, T., Nicklas, W., Rau, J., Mauder, N., Hofmann, N., Imaoka, K., Kimura, M., & Kämpfer, P. (2015). Streptobacillus notomytis sp. nov., isolated from a spinifex hopping mouse (Notomys alexis Thomas, 1922), and emended description of Streptobacillus Levaditi et al. 1925, Eisenberg et al. 2015 emend. International Journal of Systematic and Evolutionary Microbiology. 65(12), 4823-4829. https://doi.org/10.1099/ijsem.0.000654