Journal article

Authors list: Escobar, FV; Buhrke, D; Lopez, MF; Shenkutie, SM; von Horsten, S; Essen, LO; Hughes, J; Hildebrandt, P

Publication year: 2017

Pages: 1258-1265

Journal: FEBS Letters

Volume number: 591

Issue number: 9

ISSN: 1873-3468

Open access status: Bronze

DOI Link: https://doi.org/10.1002/1873-3468.12642

Publisher: Wiley

Abstract: 
The N-terminal extension (NTE) of plant phytochromes has been suggested to play a functional role in signaling photoinduced structural changes. Here, we use resonance Raman spectroscopy to study the effect of the NTE on the chromophore structure of B-type phytochromes from two evolutionarily distant plants. NTE deletion seems to have no effect on the chromophore in the inactive Pr state, but alters the torsion of the C-D ring methine bridge and the surrounding hydrogen bonding network in the physiologically active Pfr state. These changes are accompanied by a shift of the conformational equilibrium between two Pfr substates, which might affect the thermal isomerization rate of the C-D double bond and, thus, account for the effect of the NTE on the dark reversion kinetics.

Harvard Citation style: Escobar, F., Buhrke, D., Lopez, M., Shenkutie, S., von Horsten, S., Essen, L., et al. (2017) Structural communication between the chromophore-binding pocket and the N-terminal extension in plant phytochrome phyB, FEBS Letters, 591(9), pp. 1258-1265. https://doi.org/10.1002/1873-3468.12642

APA Citation style: Escobar, F., Buhrke, D., Lopez, M., Shenkutie, S., von Horsten, S., Essen, L., Hughes, J., & Hildebrandt, P. (2017). Structural communication between the chromophore-binding pocket and the N-terminal extension in plant phytochrome phyB. FEBS Letters. 591(9), 1258-1265. https://doi.org/10.1002/1873-3468.12642