Journal article

Authors list: dos Prazeres, JN; Simiqueli, AP; Pastore, GM; Linke, D; Zorn, H; Nimtz, M; Berger, RG

Publication year: 2007

Pages: 1503-1508

Journal: Fresenius Environmental Bulletin

Volume number: 16

Issue number: 11B

ISSN: 1018-4619

URL: https://www.prt-parlar.de/download_feb_2007/

Publisher: Psp

Abstract: 
Foam fractionation was used to concentrate and to purify extracellular alkaline lipases produced by Fusarium spec. in submerged cultures supplemented with Tween 80. Gas flow rate and pH-value proved to be crucial process parameters. Maximum recovery rates of lipolytic activity were obtained at 60 mL min(-1) N2 and pH 8.5. Under these conditions, > 90% of the total lipase activity was recovered in the foam with enrichment and purification factors of 8.8 and 5.9. respectively. The secreted lipases were characterized biochemically by means of IEF electrophoresis with activity staining and electrospray tandem mass spectrometry.

Harvard Citation style: dos Prazeres, J., Simiqueli, A., Pastore, G., Linke, D., Zorn, H., Nimtz, M., et al. (2007) Recovery of extracellular alkaline lipases of fusarium spec. by foam fractionation, Fresenius Environmental Bulletin, 16(11B), pp. 1503-1508. https://www.prt-parlar.de/download_feb_2007/

APA Citation style: dos Prazeres, J., Simiqueli, A., Pastore, G., Linke, D., Zorn, H., Nimtz, M., & Berger, R. (2007). Recovery of extracellular alkaline lipases of fusarium spec. by foam fractionation. Fresenius Environmental Bulletin. 16(11B), 1503-1508. https://www.prt-parlar.de/download_feb_2007/