Journal article

Authors list: Kaiser, O; Bartels, D; Bekel, T; Goesmann, A; Kespohl, S; Pühler, A; Meyer, F

Publication year: 2003

Pages: 121-133

Journal: Journal of Biotechnology

Volume number: 106

Issue number: 2-3

ISSN: 0168-1656

eISSN: 1873-4863

DOI Link: https://doi.org/10.1016/j.jbiotec.2003.08.008

Publisher: Elsevier

Abstract: 
While the sequencing of bacterial genomes has become a routine procedure at major sequencing centers, there are still a number of genome projects at small- or medium-size facilities. For these facilities a maximum of control over sequencing, assembling and finishing is essential. At the same time, facilities have to be able to co-operate at minimum costs for the overall project. We have established a pipeline for the distributed sequencing of Alcanivorax borkumensis SK2, Azoarcus sp. BH72, Clavibacter michiganensis subsp. michiganensis NCPPB382, Sorangium cellulosum So ce56 and Xanthomonas campestris pv. vesicatoria 85-10. Our pipeline relies on standard tools (e.g. PHRED/PHRAP, CAP3 and Consed/Autofinish) wherever possible, supplementing them with new tools (BioMake and BACCardI) to achieve the aims described above.

Harvard Citation style: Kaiser, O., Bartels, D., Bekel, T., Goesmann, A., Kespohl, S., Pühler, A., et al. (2003) Whole genome shotgun sequencing guided-by bioinformatics pipelines - An optimized approach for an established technique, Journal of Biotechnology, 106(2-3), pp. 121-133. https://doi.org/10.1016/j.jbiotec.2003.08.008

APA Citation style: Kaiser, O., Bartels, D., Bekel, T., Goesmann, A., Kespohl, S., Pühler, A., & Meyer, F. (2003). Whole genome shotgun sequencing guided-by bioinformatics pipelines - An optimized approach for an established technique. Journal of Biotechnology. 106(2-3), 121-133. https://doi.org/10.1016/j.jbiotec.2003.08.008