Journal article

Authors list: Thieme, F; Koebnik, R; Bekel, T; Berger, C; Boch, J; Büttner, D; Caldana, C; Gaigalat, L; Goesmann, A; Kay, S; Kirchner, O; Lanz, C; Linke, B; McHardy, AC; Meyer, F; Mittenhuber, G; Nies, DH; Niesbach-Klösgen, U; Patschkowski, T; Rückert, C; Rupp, O; Schneiker, S; Schuster, SC; Vorhölter, FJ; Weber, E; Pühler, A; Bonas, U; Bartels, D; Kaiser, O

Publication year: 2005

Pages: 7254-7266

Journal: Journal of Bacteriology

Volume number: 187

Issue number: 21

ISSN: 0021-9193

eISSN: 1098-5530

DOI Link: https://doi.org/10.1128/JB.187.21.7254-7266.2005

Publisher: American Society for Microbiology

Abstract: 
The gram-negative plant-pathogenic bacterium Xanthomonas campestris pv. vesicatoria is the causative agent of bacterial spot disease in pepper and tomato plants, which leads to economically important yield losses. This pathosystem has become a well-established model for studying bacterial infection strategies. Here, we present the whole-genome sequence of the pepper-pathogenic Xanthomonas campestris pv. vesicatoria strain 85-10, which comprises a 5.17-Mb circular chromosome and four plasmids. The genome has a high G+C content (64.75%) and signatures of extensive genome plasticity. Whole-genome comparisons revealed a gene order similar to both Xanthomonas axonopodis pv. citri and Xanthomonas campestris pv. campestris and a structure completely different from Xanthomonas oiyzae pv. oryzae. A total of 548 coding sequences (12.2%) are unique to X. campestris pv. vesicatoria. In addition to a type III secretion system, which is essential for pathogenicity, the genome of strain 85-10 encodes all other types of protein secretion systems described so far in gram-negative bacteria. Remarkably, one of the putative type IV secretion systems encoded on the largest plasmid is similar to the Icm/Dot systems of the human pathogens Legionella pneumophild and Coxiella burnetti. Comparisons with other completely sequenced plant pathogens predicted six novel type III effector proteins and several other virulence factors, including adhesins, cell wall-degrading enzymes, and extracellular polysaccharides.

Harvard Citation style: Thieme, F., Koebnik, R., Bekel, T., Berger, C., Boch, J., Büttner, D., et al. (2005) Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence, Journal of Bacteriology, 187(21), pp. 7254-7266. https://doi.org/10.1128/JB.187.21.7254-7266.2005

APA Citation style: Thieme, F., Koebnik, R., Bekel, T., Berger, C., Boch, J., Büttner, D., Caldana, C., Gaigalat, L., Goesmann, A., Kay, S., Kirchner, O., Lanz, C., Linke, B., McHardy, A., Meyer, F., Mittenhuber, G., Nies, D., Niesbach-Klösgen, U., Patschkowski, T., ...Kaiser, O. (2005). Insights into genome plasticity and pathogenicity of the plant pathogenic bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence. Journal of Bacteriology. 187(21), 7254-7266. https://doi.org/10.1128/JB.187.21.7254-7266.2005