Journalartikel

Jahr der Veröffentlichung: 2024

Zeitschrift: Gene

Bandnummer: 929

ISSN: 0378-1119

eISSN: 1879-0038

Open Access Status: Hybrid

DOI Link: https://doi.org/10.1016/j.gene.2024.148813

Verlag: Elsevier

Abstract: 
Transcription factors (TFs) have an important role in the regulation of the gene expression network. The role of TFs in the immune response of freshwater crayfish is poorly understood, but leveraging the regulatory mechanisms of immune response could augment the resistance against the invasive oomycete pathogen, Aphanomyces astaci. Previous studies indicated that the TFs CCAAT/enhancer-binding protein (C/EBP) and putative Kr & uuml;ppel homolog-1 protein (Kr-h1) might play a role in immune and stress response of the noble crayfish (Astacus astacus). Here, we aimed to further characterise these two gene products to gain a better understanding of their evolutionary origin, domain organisation and expression patterns across different crayfish tissues. Furthermore, we conducted an immune stimulation experiment to observe the potential changes in the gene expression of C/ EBP and Kr-h1 under immune challenge in different crayfish tissues. Our results showed that both C/EBP and Krh1 are closely related to other C/EBPs and Kr-h1s in Malacostraca. Gene expression analysis revealed that both TFs are present in all analysed tissues, with higher expression of C/EBP in the gills and Kr-h1 in the abdominal muscle. Immune stimulation with laminarin (mimicking beta-1-3-glucan in the oomycete cell wall) showed an activation of the crayfish immune system, with an overall increase in the total haemocyte count (THC) compared to untreated control and crayfish buffered saline (CBS) treatment. On the gene expression level, an up-regulation of the C/EBP gene was detected in the laminarin treated group in hepatopancreas and heart, while no changes were observed for the Kr-h1 gene. Our results indicate an early change in C/EBP expression in multiple tissues during immune stimulation and suggest its involvement in the immune response of the noble crayfish.

Harvard-Zitierstil: Bostjancic, L., Dragicevic, P., Bonassin, L., Francesconi, C., Tarandek, A., Schardt, L., et al. (2024) Expression of C/EBP and Kr-h1 transcription factors under immune stimulation in the noble crayfish, Gene, 929, Article 148813. https://doi.org/10.1016/j.gene.2024.148813

APA-Zitierstil: Bostjancic, L., Dragicevic, P., Bonassin, L., Francesconi, C., Tarandek, A., Schardt, L., Rutz, C., Hudina, S., Schwenk, K., Lecompte, O., & Theissinger, K. (2024). Expression of C/EBP and Kr-h1 transcription factors under immune stimulation in the noble crayfish. Gene. 929, Article 148813. https://doi.org/10.1016/j.gene.2024.148813