Journalartikel
Autorenliste: Ronzheimer, Alisa; Ringelmann, Anne E.; Morlock, Gertrud E.
Jahr der Veröffentlichung: 2024
Zeitschrift: Talanta: The International Journal of Pure and Applied Analytical Chemistry
Bandnummer: 272
ISSN: 0039-9140
eISSN: 1873-3573
Open Access Status: Hybrid
DOI Link: https://doi.org/10.1016/j.talanta.2024.125746
Verlag: Elsevier
Abstract:
High-performance thin -layer chromatography hyphenated with planar multiplex bioassays and high -resolution tandem mass spectrometry contributes to the non -target detection or even identification of active compounds in complex mixtures such as food, feed, cosmetics, commodities, and environmental samples. It can be used to discover previously unknown harmful or active substances in complex samples and to tentatively assign molecular formulas. This method is already faster than the commonly used in vitro assays along with liquid chromatographic separations, but overnight cell cultivation still prevents a planar bioassay from being performed within one day. There is also still potential for optimization in terms of sustainability. To achieve this, the planar bioassay protocols for the detection of androgen -like and estrogen -like compounds were harmonized. The successful minimization of the cell culture volume enabled accelerated cell cultivation, which allowed the bioassay to be performed within one day. This was considered a milestone achieved, as up to 23 samples per plate can now be analyzed from the start of cultivation to the biological endpoint on the same day. Doubling the substrate amount and increasing the pH of the silica gel layer led to a more sensitive and selective bioassay due to the enhanced fluorescence of the formed end -product. The faster and more sustainable bioassay protocol was applied to complex samples such as sunscreen and red wine to detect estrogen -like compounds. The developed method was validated by comparison with a standard method.
Zitierstile
Harvard-Zitierstil: Ronzheimer, A., Ringelmann, A. and Morlock, G. (2024) Fast and sustainable planar yeast-based bioassay for endocrine disruptors in complex mixtures: Start of cell cultivation to result within one day, Talanta: The International Journal of Pure and Applied Analytical Chemistry, 272, Article 125746. https://doi.org/10.1016/j.talanta.2024.125746
APA-Zitierstil: Ronzheimer, A., Ringelmann, A., & Morlock, G. (2024). Fast and sustainable planar yeast-based bioassay for endocrine disruptors in complex mixtures: Start of cell cultivation to result within one day. Talanta: The International Journal of Pure and Applied Analytical Chemistry. 272, Article 125746. https://doi.org/10.1016/j.talanta.2024.125746
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