Journal article

Authors list: Sheng, Wenjie; Zhang, Chaoyu; Mohiuddin, T. M.; Al-Rawe, Marwah; Zeppernick, Felix; Falcone, Franco H.; Meinhold-Heerlein, Ivo; Hussain, Ahmad Fawzi

Publication year: 2023

Journal: International Journal of Molecular Sciences

Volume number: 24

Issue number: 4

ISSN: 1661-6596

eISSN: 1422-0067

Open access status: Gold

DOI Link: https://doi.org/10.3390/ijms24043086

Publisher: MDPI

Abstract: 
Traditional immunohistochemistry (IHC) has already become an essential method of diagnosis and therapy in cancer management. However, this antibody-based technique is limited to detecting a single marker per tissue section. Since immunotherapy has revolutionized the antineoplastic therapy, developing new immunohistochemistry strategies to detect multiple markers simultaneously to better understand tumor environment and predict or assess response to immunotherapy is necessary and urgent. Multiplex immunohistochemistry (mIHC)/multiplex immunofluorescence (mIF), such as multiplex chromogenic IHC and multiplex fluorescent immunohistochemistry (mfIHC), is a new and emerging technology to label multiple biomarkers in a single pathological section. The mfIHC shows a higher performance in cancer immunotherapy. This review summarizes the technologies, which are applied for mfIHC, and discusses how they are employed for immunotherapy research.

Harvard Citation style: Sheng, W., Zhang, C., Mohiuddin, T., Al-Rawe, M., Zeppernick, F., Falcone, F., et al. (2023) Multiplex Immunofluorescence: A Powerful Tool in Cancer Immunotherapy, International Journal of Molecular Sciences, 24(4), Article 3086. https://doi.org/10.3390/ijms24043086

APA Citation style: Sheng, W., Zhang, C., Mohiuddin, T., Al-Rawe, M., Zeppernick, F., Falcone, F., Meinhold-Heerlein, I., & Hussain, A. (2023). Multiplex Immunofluorescence: A Powerful Tool in Cancer Immunotherapy. International Journal of Molecular Sciences. 24(4), Article 3086. https://doi.org/10.3390/ijms24043086