Journal article

Authors list: Yong, Jiawen; Li, Pingping; Mizrahi, Isaac Kably; Franzen, Rene; Groeger, Sabine; Ruf, Sabine; Gutknecht, Norbert; Marques, Marcia Martins

Publication year: 2022

Journal: Frontiers in Bioscience-Landmark

Volume number: 27

Issue number: 2

ISSN: 2768-6701

eISSN: 2768-6698

DOI Link: https://doi.org/10.31083/j.fb12702062

Publisher: IMR Press

Abstract: 
Backgrounds: Dental avulsion due to trauma, especially in young patients, is a worldwide problem, requiring tooth replacement. Delayed replantation could cause tooth loss when the cementum is severely damaged. A small number of studies has reported that photobiomodulation (PBM) therapy using Er: YAG laser irradiation activates cellular signaling responses in different cell types, resulting in a variety of favorable biological effects. The aim of this in vitro study was to evaluate the potential biostimulatory effect of low-level Er: YAG laser irradiation on the biological responses of cultured mouse cementoblasts (OCCM-30), including the mitogen-activated protein kinases (MAPKs). Methods: OCCM-30 cells were exposed to 2940 nm Er: YAG laser irradiation for 15 s at 0.34 W (pulse duration of 100 or 1000 mu s, 17 mJ/pulse) at energy densities of 1 or 2 J/cm(2). Irradiated and non-irradiated OCCM-30 cells were tested for migration (Scratch assay), proliferation (MTS assay) and functional differentiation (Alizarin Red S assay). Lumican (Lunt) and Fibromodulin (Fmod) gene expression, and activation of MAPKs, were assessed by RT-PCR and Western blotting, respectively. Results: Low-level Er: YAG laser irradiation at 2 J/cm(2) and pulse duration of 100 mu s resulted in the highest migration rate and proliferation. Moreover, the pulse duration irradiation of 100 mu s increased Lum expression. Fmod expression was increased after 1000 mu s pulse duration laser stimulation. Low-level Er: YAG laser irradiation increased the mineralization of OCCM-30 cells after 7 days and activated ERK1/2, P38 and JNK signaling. Conclusions: Low-level Er: YAG laser irradiation induces OCCM-30 cell migration, proliferation and differentiation, and activates the MAPK signaling pathway.

Harvard Citation style: Yong, J., Li, P., Mizrahi, I., Franzen, R., Groeger, S., Ruf, S., et al. (2022) Effect of Low-Level Er: YAG (2940 nm) laser irradiation on the photobiomodulation of mitogen-activated protein kinase cellular signaling pathway of rodent cementoblasts, Frontiers in bioscience. Landmark edition, 27(2), Article 062. https://doi.org/10.31083/j.fb12702062

APA Citation style: Yong, J., Li, P., Mizrahi, I., Franzen, R., Groeger, S., Ruf, S., Gutknecht, N., & Marques, M. (2022). Effect of Low-Level Er: YAG (2940 nm) laser irradiation on the photobiomodulation of mitogen-activated protein kinase cellular signaling pathway of rodent cementoblasts. Frontiers in bioscience. Landmark edition. 27(2), Article 062. https://doi.org/10.31083/j.fb12702062