Journal article

Publication year: 2021

Pages: 278-284

Journal: Urologia Internationalis

Volume number: 105

Issue number: 3-4

ISSN: 0042-1138

eISSN: 1423-0399

Open access status: Hybrid

DOI Link: https://doi.org/10.1159/000512370

Publisher: Karger Publishers

Abstract: 
Background: A catheter allowing a release of antibacterial substances such as antiseptics into the bladder could be a new way of preventing biofilm formation and subsequent catheter-associated urinary tract infections. Methods: Minimal inhibitory and bactericidal concentration (MIC/MBC) determinations in cation-adjusted Mueller-Hinton broth and artificial urine were performed for 4 antiseptics against 3 uropathogenic biofilm producers, Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis. Furthermore, effects of octenidine and polyhexanide against catheter biofilm formation were determined by quantification of biofilm-producing bacteria. Results: Sodium hypochlorite showed MIC/MBC values between 200 and 800 mg/L for all strains tested. Triclosan was efficient against E. coli and P. mirabilis (MIC <= 2.98 mg/L) but ineffective against P. aeruginosa. Octenidine and polyhexanide showed antibacterial activity against all 3 species tested (MIC 1.95-7.8 and 3.9-31.25 mg/L). Both octenidine and polyhexanide were able to prevent biofilm formation on catheter segments in a concentration dependent manner. Furthermore, adding 250 mg/L of each biocide disrupted biofilms formed by E. coli and P. mirabilis, whereas even 500 mg/L was not sufficient to completely destroy P. aeruginosa biofilms. Conclusion: Octenidine- and polyhexanide-containing antiseptics showed a broad effect against typical uropathogenic biofilm producers even in high dilutions. This study provides a basis for further investigation of the potential of octenidine and polyhexanide as prophylaxis or treatment of catheter biofilms.

Harvard Citation style: Loose, M., Naber, K., Purcell, L., Wirth, M. and Wagenlehner, F. (2021) Anti-Biofilm Effect of Octenidine and Polyhexanide on Uropathogenic Biofilm-Producing Bacteria, Urologia Internationalis, 105(3-4), pp. 278-284. https://doi.org/10.1159/000512370

APA Citation style: Loose, M., Naber, K., Purcell, L., Wirth, M., & Wagenlehner, F. (2021). Anti-Biofilm Effect of Octenidine and Polyhexanide on Uropathogenic Biofilm-Producing Bacteria. Urologia Internationalis. 105(3-4), 278-284. https://doi.org/10.1159/000512370