Journal article

Authors list: Hackstein, H.; Kalina, A.; Dorn, B.; Keil, I. S.; Baal, N.; Michel, G.; Brendel, C.; Neubauer, A.; Jakob, T.; Bein, G.

Publication year: 2021

Pages: 329-339

Journal: Clinical & Experimental Immunology

Volume number: 203

Issue number: 2

ISSN: 0009-9104

eISSN: 1365-2249

Open access status: Hybrid

DOI Link: https://doi.org/10.1111/cei.13539

Publisher: Oxford University Press

Abstract: 
Extracorporeal photopheresis (ECP) represents one of the most widespread and effective cell therapies for graft-versus-host disease and other T cell-mediated disorders. However, the key factors affecting the therapeutic efficacy of ECP remain unclear. We hypothesized that therapeutic effects are mediated by ECP-treated antigen-presenting dendritic cells (DC). To test this hypothesis, we used the experimental model of contact hypersensitivity (CHS). The ECP's therapeutic activity improved when the total cell dose of the ECP-treated cells was increased. We used different haptens during sensitization to demonstrate that the anti-inflammatory activity of ECP is antigen-specific. This confirmed the hypothesis that professional antigen-presenting cells are involved in the mode of action. Also, the ECP's therapeutic activity was abrogated by the depletion of CD11c(+) DC, which represents fewer than 1% of all the ECP-exposed cells. Finally, we confirm the critical importance of CD11c(+) DC for ECP activity by showing that only a few purified CD11c(+) DC are sufficient to mediate its therapeutic effect. The finding that ECP-treated, physiological antigen-presenting DC alone mediate antigen-specific modulation of a pathological immune response may result in better-targeted interventions when treating patients.

Harvard Citation style: Hackstein, H., Kalina, A., Dorn, B., Keil, I., Baal, N., Michel, G., et al. (2021) CD11c⁺ dendritic cells mediate antigen-specific suppression in extracorporeal photopheresis, Clinical & Experimental Immunology, 203(2), pp. 329-339. https://doi.org/10.1111/cei.13539

APA Citation style: Hackstein, H., Kalina, A., Dorn, B., Keil, I., Baal, N., Michel, G., Brendel, C., Neubauer, A., Jakob, T., & Bein, G. (2021). CD11c⁺ dendritic cells mediate antigen-specific suppression in extracorporeal photopheresis. Clinical & Experimental Immunology. 203(2), 329-339. https://doi.org/10.1111/cei.13539