Journalartikel
Autorenliste: Reder, Henrike; Taferner, Victor F.; Wittekindt, Claus; Braeuninger, Andreas; Speel, Ernst-Jan M.; Gattenloehner, Stefan; Wolf, Gregor; Klussmann, Jens P.; Wuerdemann, Nora; Wagner, Steffen
Jahr der Veröffentlichung: 2020
Seiten: 1333-1343
Zeitschrift: The Journal of Molecular Diagnostics
Bandnummer: 22
Heftnummer: 11
ISSN: 1525-1578
eISSN: 1943-7811
Open Access Status: Bronze
DOI Link: https://doi.org/10.1016/j.jmoldx.2020.08.002
Verlag: Elsevier
Abstract:
Persistent human papillomavirus (HPV) infection is associated with the development of oropharyngeal squamous cell carcinoma (OPSCC), and increasing incidences of OPSCC are reported. The generally favorable treatment outcome in patients with HPV-driven OPSCC has brought de-escalation of treatment into discussion. Nevertheless, 13% to 25% develop a relapse within two years after current standard treatment. New biomarkers are urgently required to monitor therapy response, tumor burden, and minimal residual disease during follow-up. This observational study examined 50 patients with OPSCC to investigate plasma cell-free (cf) HPV-DNA derived from tumor cells before therapy and during follow-up. Real-time quantitative PCR was applied to quantify the DNA concentration of HPV oncogenes E6 and E7. A total of 85.7% of pretreatment samples from patients with HPV-driven OPSCC (n = 28) were positive for at least one marker, and cfHPV-DNA concentration increased with tumor size. Virtually no signals were detected in HPV-negative OPSCC patients (n = 20; P <= 0.001). Patients without clinical evidence of recurrence had significantly reduced cfHPV-DNA concentrations after therapy (P <= 0.001). Conversely, cfHPV-DNA levels increased or remained above threshold in five patients who had residual disease or developed recurrence. In conclusion, plasma cfHPV-DNA detection correlates with the clinical course of disease in patients with HPV-driven OPSCC. Consequently, extensive clinical investigation should be considered if cfHPV-DNA is detected during follow-up of patients with HPV-driven OPSCC.
Zitierstile
Harvard-Zitierstil: Reder, H., Taferner, V., Wittekindt, C., Braeuninger, A., Speel, E., Gattenloehner, S., et al. (2020) Plasma Cell-Free Human Papillomavirus Oncogene E6 and E7 DNA Predicts Outcome in Oropharyngeal Squamous Cell Carcinoma, The Journal of Molecular Diagnostics, 22(11), pp. 1333-1343. https://doi.org/10.1016/j.jmoldx.2020.08.002
APA-Zitierstil: Reder, H., Taferner, V., Wittekindt, C., Braeuninger, A., Speel, E., Gattenloehner, S., Wolf, G., Klussmann, J., Wuerdemann, N., & Wagner, S. (2020). Plasma Cell-Free Human Papillomavirus Oncogene E6 and E7 DNA Predicts Outcome in Oropharyngeal Squamous Cell Carcinoma. The Journal of Molecular Diagnostics. 22(11), 1333-1343. https://doi.org/10.1016/j.jmoldx.2020.08.002
