Journalartikel
Autorenliste: Blank, Dennis; Geyer, Hildegard; Maass, Kai; Yamashita, Katsuko; Geyer, Rudolf
Jahr der Veröffentlichung: 2012
Seiten: 680-690
Zeitschrift: Analytical Biochemistry
Bandnummer: 421
Heftnummer: 2
ISSN: 0003-2697
DOI Link: https://doi.org/10.1016/j.ab.2011.11.030
Verlag: Elsevier
Abstract:
Detailed structural analysis of high molecular weight human milk oligosaccharides (HMOs) is still a challenging task. Here we present a modular strategy for a flexible de novo structural characterization of this class of molecules. The protocol combines established techniques such as separation by two-dimensional high-performance liquid chromatography with different types of mass spectrometry, exoglycosidase digestion, and linkage analysis in an individual glycan-based manner. As a proof of principle, this approach was applied to two distinct HMO isomers representing a difucosylated octaose core and a trifucosylated decaose core. Obtained data revealed the presence of one terminal Lewis A and one internal Lewis X epitope in the case of the octaose and led to the identification of this molecule as a difucosylated iso-lacto-N-octaose. The trifucosylated, doubly branched lacto-N-neo-decaose was shown to represent a new type of HMO core structure in which the branched antenna is linked to carbon atom 3 of the innermost galactosyl residue. Hence, using this analytical protocol a novel HMO structure could be defined. Our results further demonstrate that a combination of different techniques may be required for de novo structural analysis of these molecules. (C) 2011 Elsevier Inc. All rights reserved.
Zitierstile
Harvard-Zitierstil: Blank, D., Geyer, H., Maass, K., Yamashita, K. and Geyer, R. (2012) Elucidation of a novel lacto-N-decaose core structure in human milk using nonlinear analytical technique combinations, Analytical Biochemistry, 421(2), pp. 680-690. https://doi.org/10.1016/j.ab.2011.11.030
APA-Zitierstil: Blank, D., Geyer, H., Maass, K., Yamashita, K., & Geyer, R. (2012). Elucidation of a novel lacto-N-decaose core structure in human milk using nonlinear analytical technique combinations. Analytical Biochemistry. 421(2), 680-690. https://doi.org/10.1016/j.ab.2011.11.030
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