Journal article

Authors list: Blank, Dennis; Gebhardt, Sabine; Maass, Kai; Lochnit, Günter; Dotz, Viktoria; Blank, Jennifer; Geyer, Rudolf; Kunz, Clemens

Publication year: 2011

Pages: 2495-2510

Journal: Analytical and Bioanalytical Chemistry

Volume number: 401

Issue number: 8

ISSN: 1618-2642

eISSN: 1618-2650

DOI Link: https://doi.org/10.1007/s00216-011-5349-9

Publisher: Springer

Abstract: 
The structural diversity of human milk oligosaccharides (HMOs) strongly depends on the Lewis (Le) blood group status of the donor which allows a classification of these glycans into three different groups. Starting from 50 mu L of human milk, a new high-throughput, standardized, and widely automated mass spectrometric approach has been established which can be used for correlation of HMO structures with the respective Lewis blood groups on the basis of mass profiles of the entire mixture of glycans together with selected fragment ion spectra. For this purpose, the relative abundance of diagnostically relevant compositional species, such as Hex(2)Fuc(2) and Hex(3)HexNAc(1)Fuc(2), as well as the relative intensities of characteristic fragment ions obtained thereof are of key importance. For each Lewis blood group, i.e., Le(a -aEuro parts per thousand b+), Le(a + b-), and Le(a -aEuro parts per thousand b-), specific mass profile and fragment ion patterns could be thus verified. The described statistically proven classification of the derived glycan patterns may be a valuable tool for analysis and comparison of large sets of milk samples in metabolic studies. Furthermore, the outlined protocol may be used for rapid screening in clinical studies and quality control of milk samples donated to milk banks.

Harvard Citation style: Blank, D., Gebhardt, S., Maass, K., Lochnit, G., Dotz, V., Blank, J., et al. (2011) High-throughput mass finger printing and Lewis blood group assignment of human milk oligosaccharides, Analytical and Bioanalytical Chemistry, 401(8), pp. 2495-2510. https://doi.org/10.1007/s00216-011-5349-9

APA Citation style: Blank, D., Gebhardt, S., Maass, K., Lochnit, G., Dotz, V., Blank, J., Geyer, R., & Kunz, C. (2011). High-throughput mass finger printing and Lewis blood group assignment of human milk oligosaccharides. Analytical and Bioanalytical Chemistry. 401(8), 2495-2510. https://doi.org/10.1007/s00216-011-5349-9