Journalartikel

Autorenliste: Maass, Kai; Ranzinger, René; Geyer, Hildegard; von der Lieth, Claus-Wilhelm; Geyer, Rudolf

Jahr der Veröffentlichung: 2007

Seiten: 4435-4444

Zeitschrift: Proteomics: Proteomics and Systems Biology

Bandnummer: 7

Heftnummer: 24

ISSN: 1615-9853

eISSN: 1615-9861

DOI Link: https://doi.org/10.1002/pmic.200700253

Verlag: Wiley

Abstract: 
Mass spectrometric techniques are the key technology for rapid and reliable glycan analysis. However, the lack of robust, dependable, and freely available software for the (semi-) automatic annotation of mass spectra is still a severe bottleneck that hampers their rapid interpretation. In this article the" Glyco-Peakfinder" web-service is described allowing de novo determination of glycan compositions from their mass signals. Starting from a basic set of mandatory masses of glycan components, the calculation can be performed without any knowledge concerning the biological background of the sample or the fragmentation technique used. "Glyco-Peakfinder" assigns all types of fragment ions including monosaccharide cross-ring cleavage products and multiply charged ions. It provides full user control to handle modified glycans (persubstituted molecules, reducing-end modifications, glycoconjugates) and ion types. The formula applied to calculate the fragment masses and an outline of the implemented algorithm are discussed. A systematic evaluation of the dependence of all factors influencing the computation time revealed strikingly different impact of the individual calculation steps. To provide access to known carbohydrate structures a "composition search" in the open access database GLYCOSCIENCES.de can be performed. The service is available at the URL: www.eurocarbdb.org/applications/ms-tools.

Harvard-Zitierstil: Maass, K., Ranzinger, R., Geyer, H., von der Lieth, C. and Geyer, R. (2007) "Glyco-peakfinder" - de novo composition analysis of glycoconjugates, Proteomics: Proteomics and Systems Biology, 7(24), pp. 4435-4444. https://doi.org/10.1002/pmic.200700253

APA-Zitierstil: Maass, K., Ranzinger, R., Geyer, H., von der Lieth, C., & Geyer, R. (2007). "Glyco-peakfinder" - de novo composition analysis of glycoconjugates. Proteomics: Proteomics and Systems Biology. 7(24), 4435-4444. https://doi.org/10.1002/pmic.200700253