Journalartikel
Autorenliste: Lehr, Tobias; Geyer, Hildegard; Maass, Kai; Doenhoff, Michael J.; Geyer, Rudolf
Jahr der Veröffentlichung: 2007
Seiten: 82-103
Zeitschrift: Glycobiology
Bandnummer: 17
Heftnummer: 1
ISSN: 0959-6658
eISSN: 1460-2423
Open Access Status: Bronze
DOI Link: https://doi.org/10.1093/glycob/cwl048
Verlag: Oxford University Press
Abstract:
The human parasitic trematode Schistosoma mansoni has a complex life cycle that includes the freshwater snail Biomphalaria glabrata as intermediate host. Within each stage, the parasite synthesizes a wide array of glycoconjugates, exhibiting, in part, unique carbohydrate structures. In addition, the parasite expresses definitive host-like sugar epitopes, such as Lewis X determinants, supporting the concept of carbohydrate-mediated molecular mimicry as an invasion and survival strategy. In the present study, we investigated whether common carbohydrate determinants occur also at the level of the intermediate host. To this end, a structural characterization of hemolymph glycoprotein-N-glycans of B. glabrata was performed. N-glycans were released from tryptic glycopeptides and labeled with 2-aminopyridine. Sugar chains serologically cross-reacting with S. mansoni glycoconjugates were isolated by immunoaffinity chromatography using a polyclonal antiserum directed against schistosomal egg antigens and fractionated by Aleuria aurantia lectin affinity chromatography and high-performance liquid chromatography. Obtained glycans were analyzed by different mass spectrometric techniques as well as by monosaccharide constituent and linkage analysis. The results revealed a highly heterogeneous oligosaccharide pattern. Cross-reacting species represented about 5% of the total glycans and exhibited a terminal Fuc(alpha 1-3)GalNAc unit, a (1-2)-linked xylosyl residue, or both types of structural motifs. In conclusion, our study demonstrates the presence of common carbohydrate epitopes also at the level of S. mansoni and its intermediate host.
Zitierstile
Harvard-Zitierstil: Lehr, T., Geyer, H., Maass, K., Doenhoff, M. and Geyer, R. (2007) Structural characterization of N-glycans from the freshwater snail Biomphalaria glabrata cross-reacting with Schistosoma mansoni glycoconjugates, Glycobiology, 17(1), pp. 82-103. https://doi.org/10.1093/glycob/cwl048
APA-Zitierstil: Lehr, T., Geyer, H., Maass, K., Doenhoff, M., & Geyer, R. (2007). Structural characterization of N-glycans from the freshwater snail Biomphalaria glabrata cross-reacting with Schistosoma mansoni glycoconjugates. Glycobiology. 17(1), 82-103. https://doi.org/10.1093/glycob/cwl048
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