Journalartikel

Autorenliste: KLEIN, AR; BREITUNG, J; LINDER, D; STETTER, KO; THAUER, RK

Jahr der Veröffentlichung: 1993

Seiten: 213-219

Zeitschrift: Archives of Microbiology

Bandnummer: 159

Heftnummer: 3

ISSN: 0302-8933

DOI Link: https://doi.org/10.1007/BF00248474

Verlag: Springer

Abstract: 

Archaeoglobus fulgidus and Methanopyrus kandleri are both extremely thermophilic Archaea with a growth temperature optimum at 83-degrees-C and 98-degrees-C, respectively. Both Archaea contain an active N5,N10-methenyltetrahydromethanopterin cyclohydrolase. The enzyme from M. kandleri has recently been characterized. We describe here the purification and properties of the enzyme from A. fulgidus.

The cyclohydrolase from A. fulgidus was purified 180-fold to apparent homogeneity and its properties were compared with those recently published for the cyclohydrolase from M. kandleri. The two cytoplasmic enzymes were found to have very similar molecular and catalytic properties. They differed, however, significantly with respect of the effect of K2HPO4 and of other salts on the activity and the stability. The cyclohydrolase from A. fulgidus required relatively high concentrations of K2HPO4 (1 M) for optimal thermostability at 90-degrees-C but did not require salts for activity. Vice versa, the enzyme from M. kandleri was dependent on high K2HPO4 concentrations (1.5 M) for optimal activity but not for thermostability. Thus the activity and structural stability of the two thermophilic enzymes depend in a completely different way on the concentration of inorganic salts. The molecular basis for these differences are discussed.

Harvard-Zitierstil: KLEIN, A., BREITUNG, J., LINDER, D., STETTER, K. and THAUER, R. (1993) N-5, N-10-METHENYLTETRAHYDROMETHANOPTERIN CYCLOHYDROLASE FROM THE EXTREMELY THERMOPHILIC SULFATE REDUCING ARCHAEOGLOBUS-FULGIDUS - COMPARISON OF ITS PROPERTIES WITH THOSE OF THE CYCLOHYDROLASE FROM THE EXTREMELY THERMOPHILIC METHANOPYRUS-KANDLERI, Archives of Microbiology, 159(3), pp. 213-219. https://doi.org/10.1007/BF00248474

APA-Zitierstil: KLEIN, A., BREITUNG, J., LINDER, D., STETTER, K., & THAUER, R. (1993). N-5, N-10-METHENYLTETRAHYDROMETHANOPTERIN CYCLOHYDROLASE FROM THE EXTREMELY THERMOPHILIC SULFATE REDUCING ARCHAEOGLOBUS-FULGIDUS - COMPARISON OF ITS PROPERTIES WITH THOSE OF THE CYCLOHYDROLASE FROM THE EXTREMELY THERMOPHILIC METHANOPYRUS-KANDLERI. Archives of Microbiology. 159(3), 213-219. https://doi.org/10.1007/BF00248474

Schlagwörter

  

  

  

1 von 2

  

  

  

---

• ARCHAEBACTERIA
• CELL-EXTRACTS
• FORMYLMETHANOFURAN
• HYPERTHERMOPHILES
• METHANOBACTERIUM THERMOAUTOTROPHICUM