Journalartikel
Autorenliste: HEDDERICH, R; ALBRACHT, SPJ; LINDER, D; KOCH, J; THAUER, RK
Jahr der Veröffentlichung: 1992
Seiten: 65-68
Zeitschrift: FEBS Letters
Bandnummer: 298
Heftnummer: 1
ISSN: 0014-5793
DOI Link: https://doi.org/10.1016/0014-5793(92)80023-A
Verlag: Wiley
Abstract:
The methylviologen-reducing hydrogenase operon of Methanobacterium thermoautotrophicum contains an open reading frame, mvhB, the product of which was predicted to have a molecular weight of 44 kDa and to contain as many as 48 iron atoms in 12 [4Fe-4S] clusters, and was therefore suggested to be a polyferredoxin. We have now, for the first time, isolated this polyferredoxin. Its identity with the mvhB gene product was evidenced by a comparison of the N-terminal amino acid sequence. The dark-brown protein of apparent molecular weight 44 kDa was found to contain 53 mol Fe and 43 mol acid-labile sulfur per mol. The UV/visible spectrum showed two maxima at 280 nm and 390 nm, and a shoulder at 308 nm. The A390/A280 ratio was 0,73. The molar extinction coefficient at 390 nm was 170,000 M-1 cm-1. In the dithionite reduced state the protein displayed an EPR spectrum like that of [4Fe-4S] clusters. The results indicate that the mvhB gene product is indeed a polyferredoxin.
Zitierstile
Harvard-Zitierstil: HEDDERICH, R., ALBRACHT, S., LINDER, D., KOCH, J. and THAUER, R. (1992) ISOLATION AND CHARACTERIZATION OF POLYFERREDOXIN FROM METHANOBACTERIUM-THERMOAUTOTROPHICUM - THE MVHB GENE-PRODUCT OF THE METHYLVIOLOGEN-REDUCING HYDROGENASE OPERON, FEBS Letters, 298(1), pp. 65-68. https://doi.org/10.1016/0014-5793(92)80023-A
APA-Zitierstil: HEDDERICH, R., ALBRACHT, S., LINDER, D., KOCH, J., & THAUER, R. (1992). ISOLATION AND CHARACTERIZATION OF POLYFERREDOXIN FROM METHANOBACTERIUM-THERMOAUTOTROPHICUM - THE MVHB GENE-PRODUCT OF THE METHYLVIOLOGEN-REDUCING HYDROGENASE OPERON. FEBS Letters. 298(1), 65-68. https://doi.org/10.1016/0014-5793(92)80023-A
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