Journal article
Authors list: ORLICKY, J; RUSCAK, M; RUSCAKOVA, D; HAGER, H
Publication year: 1981
Pages: 323-328
Journal: Physiologia Bohemoslovaca
Volume number: 30
Issue number: 4
ISSN: 0014-1291
Publisher: Academia Scientiarum Bohemoslovaca
Abstract:
Activity of certain key enzymes involved in glutamic acid metabolism was studied in purified bovine brain mitochondria and in mitochondrial subfractions separated in a discontinuous 1.2-1.6 mol/l sucrose gradient. Alanine aminotransferase and glutamate dehydrogenase were matrix enzymes, and aspartate aminotransferase was associated with the inner mitochondrial membranes. After the purified mitochondria were separated into 5 subfractions, aspartate aminotransferase and NAD+-dependent isocitrate dehydrogenase were bound to the lighter mitochondrial subfractions settling at the 1.4-1.5 mol/l sucrose boundary while alanine aminotransferase, 4-aminobutyrate transaminase and glutamate dehydrogenase were associated with the heavier subfractions settling below 1.4 mol/l sucrose. The highest specific activity of the given enzymes was found in the subfraction settling at the 1.4-1.5 mol/l sucrose boundary, the only exception being alanine aminotransferase activity, whose maximum was found in the subfractions settling in 1.5 and 1.6 mol/l sucrose. Alanine aminotransferase, in conjunction with glutamate dehydrogenase, is apparently linked to NH3 binding and to the oxidation of reduced adenine nucleotides. Alanine aminotransferase is presumed to have the function of transporting glutamate from the mitochondria to the extramitochondrial space.
Citation Styles
Harvard Citation style: ORLICKY, J., RUSCAK, M., RUSCAKOVA, D. and HAGER, H. (1981) ALANINE AMINOTRANSFERASE AND SOME OTHER ENZYMES IN DIFFERENT POPULATIONS OF FREE BRAIN CORTEX MITOCHONDRIA, PHYSIOLOGIA BOHEMOSLOVACA, 30(4), pp. 323-328
APA Citation style: ORLICKY, J., RUSCAK, M., RUSCAKOVA, D., & HAGER, H. (1981). ALANINE AMINOTRANSFERASE AND SOME OTHER ENZYMES IN DIFFERENT POPULATIONS OF FREE BRAIN CORTEX MITOCHONDRIA. PHYSIOLOGIA BOHEMOSLOVACA. 30(4), 323-328.
