Journal article

Authors list: Meyer, Daniel; Hörnlein, Sophia; Rein, Dietrich; Morlock, Gertrud E.

Publication year: 2025

Journal: Analytical and Bioanalytical Chemistry

ISSN: 1618-2642

eISSN: 1618-2650

Open access status: Gold

DOI Link: https://doi.org/10.1007/s00216-025-05894-0

Publisher: Springer

Abstract: 
Preschool-aged children and non-pregnant women of reproductive age particularly in countries of a lower socio-demographic index may experience insufficient dietary vitamin A. Fortifying staple foods such as edible oils with vitamin A is a useful approach to reduce malnutrition. To enable better quality oil fortification, producers need reliable and inexpensive tools for assessment of the vitamin A contents of their products. A simple reversed-phase high-performance thin-layer chromatography fluorescence detection (RP-HPTLC-FLD) method was developed and studied for the quantification of vitamin A palmitate (VAP) in five edible plant oils. The challenging matrices of non-refined palm kernel oil and soybean oil, which are known to interfere with the accuracy of some easy-to-use test kits, were separated from VAP using the new RP-HPTLC-FLD method. The standard addition method provided good accuracy and acceptable calibration curves (coefficients of determination R2 between 0.9874 and 0.9999) even for manual application. The developed RP-HPTLC-FLD method proved simple, robust, sustainable, cost-efficient, and suitable for low-tech (minimum equipment) on-site analysis.

Harvard Citation style: Meyer, D., Hörnlein, S., Rein, D. and Morlock, G. (2025) Proposed RP-HPTLC-FLD kit for analysis of vitamin A palmitate in edible oils, Analytical and Bioanalytical Chemistry. https://doi.org/10.1007/s00216-025-05894-0

APA Citation style: Meyer, D., Hörnlein, S., Rein, D., & Morlock, G. (2025). Proposed RP-HPTLC-FLD kit for analysis of vitamin A palmitate in edible oils. Analytical and Bioanalytical Chemistry. https://doi.org/10.1007/s00216-025-05894-0