Journal article

Authors list: Eder, K; Reichlmayr-Lais, AM; Kirchgeßner, M

Publication year: 1992

Pages: 55-67

Journal: Journal of Chromatography A

Volume number: 607

Issue number: 1

ISSN: 0021-9673

DOI Link: https://doi.org/10.1016/0021-9673(92)87054-C

Publisher: Elsevier

Abstract: 

The methanolysis of small amounts of purified phosphoglycerides and sphingomyelin was studied and a quantitative comparison of five methods for the methanolysis of standard phosphoglycerides was made. These methods were based on methanolysis with boron trifluoride-methanol, methanolic sodium methoxide (at ambient temperature and with heating) and methanolic sulphuric acid. A further method was based on saponification with methanolic sodium hydroxide and subsequent esterification with boron trifluoride-methanol. Under the experimental conditions, only the sodium methoxide-catalysed method at ambient temperature gave complete methanolysis of phosphoglycerides. For methanolysis of sphingomyelin, boron trifluoride-methanol, methanolic sulphuric acid and methanolic hydrochloric acid were used. It was found that complete methanolysis of sphingomyelin takes 15 h at 90-degrees-C. Based on these results, procedures for the methanolysis of phosphoglycerides and sphingomyelin separated by high-performance liquid chromatography are presented.

Harvard Citation style: Eder, K., Reichlmayr-Lais, A. and Kirchgeßner, M. (1992) Studies on the methanolysis of small amounts of purified phospholipids for gas chromatographic analysis of fatty acid methyl esters, Journal of Chromatography A, 607(1), pp. 55-67. https://doi.org/10.1016/0021-9673(92)87054-C

APA Citation style: Eder, K., Reichlmayr-Lais, A., & Kirchgeßner, M. (1992). Studies on the methanolysis of small amounts of purified phospholipids for gas chromatographic analysis of fatty acid methyl esters. Journal of Chromatography A. 607(1), 55-67. https://doi.org/10.1016/0021-9673(92)87054-C