Journal article

Authors list: Eder, K; Reichlmayr-Lais, AM; Kirchgeßner, M

Publication year: 1992

Pages: 33-42

Journal: Journal of Chromatography A

Volume number: 598

Issue number: 1

ISSN: 0021-9673

DOI Link: https://doi.org/10.1016/0021-9673(92)85111-6

Publisher: Elsevier

Abstract: 

A method for the simultaneous determination of amounts of major phospholipid classes and their fatty acid composition in erythrocyte membranes is described. The method consists in extraction of phospholipids from erythrocyte membranes, separation of phospholipid classes by high-performance liquid chromatography, methylation of phospholipids and determination of phospholipid-bound fatty acids by capillary gas chromatography. The amounts of phospholipid classes are calculated from the total weight of phospholipid-bound fatty acids and their average molecular weights. The method was applied to erythrocytes from rats. The results show that the method is reproducible and is useful for the determination of amounts of phospholipid classes and their fatty acid composition in small blood samples.

Harvard Citation style: Eder, K., Reichlmayr-Lais, A. and Kirchgeßner, M. (1992) Simultaneous determination of amounts of major phospholipid classes and their fatty acid composition in erythrocyte membranes using high-performance liquid chromatography and gas chromatography, Journal of Chromatography A, 598(1), pp. 33-42. https://doi.org/10.1016/0021-9673(92)85111-6

APA Citation style: Eder, K., Reichlmayr-Lais, A., & Kirchgeßner, M. (1992). Simultaneous determination of amounts of major phospholipid classes and their fatty acid composition in erythrocyte membranes using high-performance liquid chromatography and gas chromatography. Journal of Chromatography A. 598(1), 33-42. https://doi.org/10.1016/0021-9673(92)85111-6