Marine Myxobacteria as a Source of Antibiotics-Comparison of Physiology, Polyketide-Type Genes and Antibiotic Production of Three New Isolates of Enhygromyxa salina - Research portal of Justus Liebig University Giessen:

Journal article

Marine Myxobacteria as a Source of Antibiotics-Comparison of Physiology, Polyketide-Type Genes and Antibiotic Production of Three New Isolates of Enhygromyxa salina

Authors list: Schäberle, TF; Goralski, E; Neu, E; Erol, O; Hölzl, G; Dormann, P; Bierbaum, G; König, GM

Publication year: 2010

Pages: 2466-2479

Journal: Marine Drugs

Volume number: 8

Issue number: 9

ISSN: 1660-3397

DOI Link: https://doi.org/10.3390/md8092466

Publisher: MDPI

Abstract:
Three myxobacterial strains, designated SWB004, SWB005 and SWB006, were obtained from beach sand samples from the Pacific Ocean and the North Sea. The strains were cultivated in salt water containing media and subjected to studies to determine their taxonomic status, the presence of genes for the biosynthesis of polyketides and antibiotic production. 16S rDNA sequence analysis revealed the type strain Enhygromyxa salina SHK-1(T) as their closest homolog, displaying between 98% (SWB005) and 99% (SWB004 and SWB006) sequence similarity. All isolates were rod-shaped cells showing gliding motility and fruiting body formation as is known for myxobacteria. They required NaCl for growth, with an optimum concentration of around 2% [w/v]. The G + C-content of genomic DNA ranged from 63.0 to 67.3 mol%. Further, the strains were analyzed for their potential to produce polyketide-type structures. PCR amplified ketosynthase-like gene fragments from all three isolates enhances the assumption that these bacteria produce polyketides. SWB005 was shown to produce metabolites with prominent antibacterial activity, including activity towards methicillin resistant Staphylococcus aureus (MRSA) and Staphylococcus epidermidis (MRSE).

Authors/Editors

- Uni.-Prof. Dr. Till Friedrich Schäberle

Citation Styles

Harvard Citation style: Schäberle, T., Goralski, E., Neu, E., Erol, O., Hölzl, G., Dormann, P., et al. (2010) Marine Myxobacteria as a Source of Antibiotics-Comparison of Physiology, Polyketide-Type Genes and Antibiotic Production of Three New Isolates of Enhygromyxa salina, Marine Drugs, 8(9), pp. 2466-2479. https://doi.org/10.3390/md8092466

APA Citation style: Schäberle, T., Goralski, E., Neu, E., Erol, O., Hölzl, G., Dormann, P., Bierbaum, G., & König, G. (2010). Marine Myxobacteria as a Source of Antibiotics-Comparison of Physiology, Polyketide-Type Genes and Antibiotic Production of Three New Isolates of Enhygromyxa salina. Marine Drugs. 8(9), 2466-2479. https://doi.org/10.3390/md8092466