Journal article

Authors list: Eder, K; Reichlmayr-Lais, AM; Kirchgeßner, M

Publication year: 1989

Pages: 151-159

Journal: Journal of trace elements and electrolytes in health and disease : analytical methods, metabolism ..

Volume number: 3

Issue number: 3

ISSN: 0931-2838

Publisher: De Gruyter

Abstract: 

The present paper is concerned with Na-K-ATPase. In the first part it deals with the effect of such factors as Na+, K+, Mg2+, ATP, pH on activity investigated using a soluble Na-K-ATPase from porcine cerebral cortex. In the second part a method for assaying red blood cell Na-K-ATPase is described. This method consists of specific steps for washing and preparation of red cell membranes, pretreatment of Na-K-ATPase with sodium dodecyl sulfate (SDS) and bovine serum albumin (BSA) and determination of enzymatic released inorganic phosphate. Na-K-ATPase prepared and treated in this way shows a higher activity than the conventionally prepared form of enzyme, as latent enzyme sites are exposed by the SDS treatment. The kinetic behaviour of Na-K-ATPase prepared and assayed by using this method is characterized and compared with that of soluble Na-K-ATPase.

Harvard Citation style: Eder, K., Reichlmayr-Lais, A. and Kirchgeßner, M. (1989) Studies on the determination of Na-K-ATPase in red blood cell membranes, Journal of trace elements and electrolytes in health and disease : analytical methods, metabolism .., 3(3), pp. 151-159

APA Citation style: Eder, K., Reichlmayr-Lais, A., & Kirchgeßner, M. (1989). Studies on the determination of Na-K-ATPase in red blood cell membranes. Journal of trace elements and electrolytes in health and disease : analytical methods, metabolism ... 3(3), 151-159.