Journal article

Authors list: Eder, K; Reichlmayr-Lais, AM; Kirchgeßner, M

Publication year: 1993

Pages: 93-104

Journal: Clinica Chimica Acta

Volume number: 219

Issue number: 1-2

ISSN: 0009-8981

DOI Link: https://doi.org/10.1016/0009-8981(93)90200-N

Publisher: Elsevier

Abstract: 
The effects of choice of solvent and period of extraction on recovery of phospholipid classes extracted from rat erythrocyte membranes were investigated. For extraction of phosphatidylserine (PS) the best recovery was achieved using chloroform-methanol (2: 1, v/v), for diacyl phosphatidylethanolamine (PE) acetone-isopropanol (3:2, v/v), and for extraction of plasmalogen PE, phosphatidylcholine (PC) and sphingomyelin (SM) using hexane-isopropanol (3:2, v/v). Using hexane-isopropanol (3:2, v/v), boiling during the extraction increased the recovery of some of the individual phospholipid classes whereas mechanical treatment of the sample had only a slight effect. Furthermore, the fatty acid composition of PS and PE varied most with different extraction conditions and the phospholipid classes PC and SM were extracted more readily from rat erythrocyte membranes than PS, diacyl and plasmalogen PE.

Harvard Citation style: Eder, K., Reichlmayr-Lais, A. and Kirchgeßner, M. (1993) Studies on the extraction of phospholipids from erythrocyte membranes in the rat, Clinica Chimica Acta, 219(1-2), pp. 93-104. https://doi.org/10.1016/0009-8981(93)90200-N

APA Citation style: Eder, K., Reichlmayr-Lais, A., & Kirchgeßner, M. (1993). Studies on the extraction of phospholipids from erythrocyte membranes in the rat. Clinica Chimica Acta. 219(1-2), 93-104. https://doi.org/10.1016/0009-8981(93)90200-N