Journal article

Authors list: Lick, S; Kröckel, L; Wibberg, D; Winkler, A; Blom, J; Goesmann, A; Kalinowski, J

Publication year: 2020

Pages: 292-301

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 70

Issue number: 1

ISSN: 1466-5026

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.003751

Publisher: Microbiology Society

Abstract: 
Two Pseudomonas isolates derived independently from raw refrigerated processing meat of bovine origin intended for the manufacture of Bologna-type cooked sausage could be distinguished from other known species in subsequent phylogenetic analyses. Comparison of the complete rpoB gene sequences in combination with nearly complete 16S rRNA gene sequences revealed a separate branch within the Pseudomonas fragi group. In further analyses, comprising phenotypic and chemotaxonomic characterization as well as average nucleotide identity (ANI) values obtained from the draft genome assemblies, the two isolates could be distinguished from all so far published closely related species. The closest relative was P. fragi DSM 3456(T) with ANI values of about 90.2%. Other close Pseudomonas neighbours were P. psychrophila DSM 17535(T) (86.5%), P. deceptionensis DSM 26521(T) (86.4 %), P. versuta DSM 101070(T) (83.8%), P. taetrolens DSM 21104(T) (83.2%), P. weihenstephanensis DSM 29166(T) (82.3%), P. helleri DSM 29165(T) (82.7%) and P. lundensis DSM 6252(T) (81.9 %). The G+C contents of isolates TH39(T) and TH26 were both 58.2 mol%. The major cellular lipids of strain TH39(T) were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major quinone was Q9 with small amounts of Q8. Based on these data, the isolates TH39(T) and TH26 (=DSM 107389=LMG 30831) represent a novel species within the genus Pseudomonas, for which the name Pseudomonas bubulae sp. nov. is proposed. The type strain is TH39(T) (=DSM 107390(T)=LMG 30830(T))

Harvard Citation style: Lick, S., Kröckel, L., Wibberg, D., Winkler, A., Blom, J., Goesmann, A., et al. (2020) Pseudomonas bubulae sp. nov., isolated from beef, International Journal of Systematic and Evolutionary Microbiology, 70(1), pp. 292-301. https://doi.org/10.1099/ijsem.0.003751

APA Citation style: Lick, S., Kröckel, L., Wibberg, D., Winkler, A., Blom, J., Goesmann, A., & Kalinowski, J. (2020). Pseudomonas bubulae sp. nov., isolated from beef. International Journal of Systematic and Evolutionary Microbiology. 70(1), 292-301. https://doi.org/10.1099/ijsem.0.003751