Journal article

Authors list: Lick, S; Kröckel, L; Wibberg, D; Winkler, A; Blom, J; Bantleon, A; Goesmann, A; Kalinowski, J

Publication year: 2020

Pages: 1528-1540

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 70

Issue number: 3

ISSN: 1466-5026

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.003928

Publisher: Microbiology Society

Abstract: 

During investigations of spoilage-associated meat microbiota, Pseudomonas isolates were found in two different laboratories showing highest similarities to Pseudomonas lactis DSM 29167(T), Pseudomonas paralactis DSM 29164(T) and Pseudomonas azotoformans DSM 18862(T) based on 165 rRNA gene sequence comparisons. Phylogenetic analysis of the complete rpoB gene sequences of isolates B4-1(T) and SpeckC indicated a separate branch with 99.0 and 99.1% identity, respectively, to their closest relative (P lactis DSM 29167(T)). Further phenotypic and chemotaxonomic characterizations, as well as average nucleotide identity (ANIb) values obtained from the draft genomes, revealed that these isolates could be considered as representing a novel species, with ANIb values of around 94 and 90% with their closest relatives P lactis and P paralactis. Other related species showed ANIb values below 90%, including Pseudomonas libanensis DSM 17149(T), Pseudomonas synxantha DSM 18928(T), Pseudomonas orientalis DSM 17489(T), Pseudomonas veronii DSM 11331(T) and P azotoformans DSM 18862(T). Genome-to-genome distance calculations between B4-1(T) and its closest relative, P lactis DSM 29167(T), showed 62.6% relatedness. The G+C contents of B4-1(T) and SpeckC were 59.8 and 59.9 mol%, respectively. The major cellular lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major quinone was 09. Based on these data, the new species Pseudomonas carnis sp. nov. is proposed, the type strain is B4-1(T) (=DSM 107652(T) =LMG 30892(T)); a second strain is SpeckC (=DSM 107651=LMG 30893).

Harvard Citation style: Lick, S., Kröckel, L., Wibberg, D., Winkler, A., Blom, J., Bantleon, A., et al. (2020) Pseudomonas carnis sp. nov., isolated from meat, International Journal of Systematic and Evolutionary Microbiology, 70(3), pp. 1528-1540. https://doi.org/10.1099/ijsem.0.003928

APA Citation style: Lick, S., Kröckel, L., Wibberg, D., Winkler, A., Blom, J., Bantleon, A., Goesmann, A., & Kalinowski, J. (2020). Pseudomonas carnis sp. nov., isolated from meat. International Journal of Systematic and Evolutionary Microbiology. 70(3), 1528-1540. https://doi.org/10.1099/ijsem.0.003928