Journal article

Authors list: Franco, A; Rückert, C; Blom, J; Busche, T; Reichert, J; Schubert, P; Goesmann, A; Kalinowski, J; Wilke, T; Kämpfer, P; Glaeser, SP

Publication year: 2020

Pages: 126123-

Journal: Systematic and Applied Microbiology

Volume number: 43

Issue number: 5

ISSN: 0723-2020

DOI Link: https://doi.org/10.1016/j.syapm.2020.126123

Publisher: Elsevier

Abstract: 
The aim of the study was to characterise the diversity and niche-specific colonization of Vibrio spp. in a marine aquaria system by a cultivation-dependent approach. A total of 53 Vibrio spp. isolates were cultured from different ecological niches in a marine aquarium including microplastic (MP) and sandy sediment particles (12 weeks after added sterile to the system), detritus, and the surrounding aquarium water. Based on the 16S rRNA gene sequence phylogeny and multilocus sequence analysis (MLSA) the isolates were assigned to seven different phylotypes. Six phylotypes were identified by high probability to the species level. The highest phylotype diversity was cultured from detritus and water (six out of seven phylotypes), while only two phylotypes were cultured from MP and sediment particles. Genomic fingerprinting indicated an even higher genetic diversity of Vibrio spp. at the strain (genotype) level. Again, the highest diversity of genotypes was recovered from detritus and water while only few partially particle-type specific genotypes were cultured from MP and sediment particles. Phylotype V-2 formed an independent branch in the MLSA tree and could not be assigned to a described Vibrio species. Isolates of this phylotype showed highest 16S rRNA gene sequence similarity to type strains of Vibrio japonicus (98.5%) and Vibrio caribbeanicus (98.4%). A representative isolate, strain THAF100(T), was characterised by a polyphasic taxonomic approach and Vibrio aquimaris sp. nov., with strain THAF100(T) (=DSM 109633(T )=LMG 31434(T) = CIP 111709(T)) as type strain, is proposed as novel species. (C) 2020 Elsevier GmbH. All rights reserved.

Harvard Citation style: Franco, A., Rückert, C., Blom, J., Busche, T., Reichert, J., Schubert, P., et al. (2020) High diversity of Vibrio spp. associated with different ecological niches in a marine aquaria system and description of Vibrio aquimaris sp. nov, Systematic and Applied Microbiology, 43(5), p. 126123. https://doi.org/10.1016/j.syapm.2020.126123

APA Citation style: Franco, A., Rückert, C., Blom, J., Busche, T., Reichert, J., Schubert, P., Goesmann, A., Kalinowski, J., Wilke, T., Kämpfer, P., & Glaeser, S. (2020). High diversity of Vibrio spp. associated with different ecological niches in a marine aquaria system and description of Vibrio aquimaris sp. nov. Systematic and Applied Microbiology. 43(5), 126123. https://doi.org/10.1016/j.syapm.2020.126123