Journalartikel

Autorenliste: Edelmann, D; Berghoff, BA

Jahr der Veröffentlichung: 2019

Seiten: 14256-

Zeitschrift: Scientific Reports

Bandnummer: 9

ISSN: 2045-2322

Open Access Status: Gold

DOI Link: https://doi.org/10.1038/s41598-019-50668-1

Verlag: Nature Research

Abstract: 
Induction of growth stasis by bacterial toxins from chromosomal toxin-antitoxin systems is suspected to favor formation of multidrug-tolerant cells, named persisters. Recurrent infections are often attributed to resuscitation and regrowth of persisters upon termination of antibiotic therapy. Several lines of evidence point to oxidative stress as a crucial factor during the persister life cycle. Here, we demonstrate that the membrane-depolarizing type I toxins TisB, DinQ, and HokB have the potential to provoke reactive oxygen species formation in Escherichia coli. More detailed work with TisB revealed that mainly superoxide is formed, leading to activation of the SoxRS regulon. Deletion of the genes encoding the cytoplasmic superoxide dismutases SodA and SodB caused both a decline in TisB-dependent persisters and a delay in persister recovery upon termination of antibiotic treatment. We hypothesize that expression of depolarizing toxins during the persister formation process inflicts an oxidative challenge. The ability to counteract oxidative stress might determine whether cells will survive and how much time they need to recover from dormancy.

Harvard-Zitierstil: Edelmann, D. and Berghoff, B. (2019) Type I toxin-dependent generation of superoxide affects the persister life cycle of Escherichia coli, Scientific Reports, 9, p. 14256. https://doi.org/10.1038/s41598-019-50668-1

APA-Zitierstil: Edelmann, D., & Berghoff, B. (2019). Type I toxin-dependent generation of superoxide affects the persister life cycle of Escherichia coli. Scientific Reports. 9, 14256. https://doi.org/10.1038/s41598-019-50668-1