Journal article

Authors list: Nielsen, AF; Bindereif, A; Bozzoni, I; Hanan, M; Hansen, TB; Irimia, M; Kadener, S; Kristensen, LS; Legnini, I; Morlando, M; Jarlstad Olesen, MT; Pasterkamp, RJ; Preibisch, S; Rajewsky, N; Suenkel, C; Kjems, J

Publication year: 2022

Pages: 1208-1220

Journal: Nature Methods

Volume number: 19

ISSN: 1548-7091

Open access status: Green

DOI Link: https://doi.org/10.1038/s41592-022-01487-2

Publisher: Nature Research

Abstract: 
Circular RNAs (circRNAs) are formed in all domains of life and via different mechanisms. There has been an explosion in the number of circRNA papers in recent years; however, as a relatively young field, circRNA biology has an urgent need for common experimental standards for isolating, analyzing, expressing and depleting circRNAs. Here we propose a set of guidelines for circRNA studies based on the authors' experience. This Perspective will specifically address the major class of circRNAs in Eukarya that are generated by a spliceosome-catalyzed back-splicing event. We hope that the implementation of best practice principles for circRNA research will help move the field forward and allow a better functional understanding of this fascinating group of RNAs.

Harvard Citation style: Nielsen, A., Bindereif, A., Bozzoni, I., Hanan, M., Hansen, T., Irimia, M., et al. (2022) Best practice standards for circular RNA research, Nature Methods, 19, pp. 1208-1220. https://doi.org/10.1038/s41592-022-01487-2

APA Citation style: Nielsen, A., Bindereif, A., Bozzoni, I., Hanan, M., Hansen, T., Irimia, M., Kadener, S., Kristensen, L., Legnini, I., Morlando, M., Jarlstad Olesen, M., Pasterkamp, R., Preibisch, S., Rajewsky, N., Suenkel, C., & Kjems, J. (2022). Best practice standards for circular RNA research. Nature Methods. 19, 1208-1220. https://doi.org/10.1038/s41592-022-01487-2