Journal article

Authors list: Rick, T; Kreiling, V; Höing, A; Fiedler, S; Glatter, T; Steinchen, W; Hochberg, G; Bähre, H; Seifert, R; Bange, G; Knauer, SK; Graumann, PL; Thormann, KM

Publication year: 2022

Pages: 35-

Journal: npj Biofilms and Microbiomes

Volume number: 8

Issue number: 1

eISSN: 2055-5008

Open access status: Gold

DOI Link: https://doi.org/10.1038/s41522-022-00297-w

Publisher: Nature Research

Abstract: 
In bacteria, the monopolar localization of enzymes and protein complexes can result in a bimodal distribution of enzyme activity between the dividing cells and heterogeneity of cellular behaviors. In Shewanella putrefaciens, the multidomain hybrid diguanylate cyclase/phosphodiesterase PdeB, which degrades the secondary messenger c-di-GMP, is located at the flagellated cell pole. Here, we show that direct interaction between the inactive diguanylate cyclase (GGDEF) domain of PdeB and the FimV domain of the polar landmark protein HubP is crucial for full function of PdeB as a phosphodiesterase. Thus, the GGDEF domain serves as a spatially controlled on-switch that effectively restricts PdeBs activity to the flagellated cell pole. PdeB regulates abundance and activity of at least two crucial surface-interaction factors, the BpfA surface-adhesion protein and the MSHA type IV pilus. The heterogeneity in c-di-GMP concentrations, generated by differences in abundance and timing of polar appearance of PdeB, orchestrates the population behavior with respect to cell-surface interaction and environmental spreading.

Harvard Citation style: Rick, T., Kreiling, V., Höing, A., Fiedler, S., Glatter, T., Steinchen, W., et al. (2022) GGDEF domain as spatial on-switch for a phosphodiesterase by interaction with landmark protein HubP, npj Biofilms and Microbiomes, 8(1), Article 35. p. 35. https://doi.org/10.1038/s41522-022-00297-w

APA Citation style: Rick, T., Kreiling, V., Höing, A., Fiedler, S., Glatter, T., Steinchen, W., Hochberg, G., Bähre, H., Seifert, R., Bange, G., Knauer, S., Graumann, P., & Thormann, K. (2022). GGDEF domain as spatial on-switch for a phosphodiesterase by interaction with landmark protein HubP. npj Biofilms and Microbiomes. 8(1), Article 35, 35. https://doi.org/10.1038/s41522-022-00297-w