Journal article

Authors list: von Bredow, YM; von Bredow, CR; Trenczek, TE

Publication year: 2020

Pages: 103722-

Journal: Developmental & Comparative Immunology: Ontogeny, Phylogeny, Aging

Volume number: 111

ISSN: 0145-305X

eISSN: 1879-0089

DOI Link: https://doi.org/10.1016/j.dci.2020.103722

Publisher: Elsevier

Abstract: 
With a set of haemocyte specific markers novel findings on haematopoiesis in the Manduca sexta embryo are presented. We identify a hitherto unknown paired haematopoietic cluster, the abdominal haemocyte cluster in abdominal segment 7 (A7-HCC). These clusters are localised at distinct positions and are established at around katatrepsis. Later in embryogenesis, the A7-HCCs disintegrate, thereby releasing numerous embryonic plasmatocytes which disperse both anteriorly and posteriorly. These cells follow stereotypic migration routes projecting anteriorly. The thoracic larval haematopoietic organs are established at around midembryogenesis. We identify embryonic oenocytoids in the M. sexta embryo for the first time. They appear in the head region roughly at the same time as the A7-HCCs occur and successively disperse in the body cavity during development. Localisation of the prophenoloxidase (proPO) mRNA and of the proPO protein are identical. Morphological, cytometric and antigenic traits show three independently generated haemocyte types during embryogenesis.

Harvard Citation style: von Bredow, Y., von Bredow, C. and Trenczek, T. (2020) A novel site of haematopoiesis and appearance and dispersal of distinct haemocyte types in the Manduca sexta embryo (Insecta, Lepidoptera), Developmental & Comparative Immunology: Ontogeny, Phylogeny, Aging, 111, Article 103722. p. 103722. https://doi.org/10.1016/j.dci.2020.103722

APA Citation style: von Bredow, Y., von Bredow, C., & Trenczek, T. (2020). A novel site of haematopoiesis and appearance and dispersal of distinct haemocyte types in the Manduca sexta embryo (Insecta, Lepidoptera). Developmental & Comparative Immunology: Ontogeny, Phylogeny, Aging. 111, Article 103722, 103722. https://doi.org/10.1016/j.dci.2020.103722