Journal article

Authors list: von Bredow, CR; von Bredow, YM; Trenczek, TE

Publication year: 2021

Pages: 103858-

Journal: Developmental & Comparative Immunology: Ontogeny, Phylogeny, Aging

Volume number: 115

ISSN: 0145-305X

eISSN: 1879-0089

DOI Link: https://doi.org/10.1016/j.dci.2020.103858

Publisher: Elsevier

Abstract: 
Haematopoietic organs (HOs) in Lepidoptera are widely recognised as the source for at least two haemocyte types. With new specific markers for oenocytoids and spherule cells and a method to identify prohaemocytes, the haemocytes formed in and released by the HOs of Manduca sexta are characterised. Differentiation of HO-cells to haemocytes other than plasmatocytes and prohaemocytes neither occurs in the organ itself nor in cells released in vitro by the HOs. Differential labelling patterns evidence the existence of plasmatocyte subpopulations and prohaemocytes, which might represent a gradual differentiation of haemocytes within the organs. Prohaemocytes can be identified by PNA-labelling of the cell membrane. These prohaemocytes are found in circulation and in the HOs and are released by the organs. Circulating prohaemocytes possess characteristics for granular cells, plasmatocytes or oenocytoids while HO derived prohaemocytes share characteristics only with plasmatocytes. Ablation of the HOs diminishes the plasmatocyte and prohaemocyte number, indicating a true larval haematopoietic function.

Harvard Citation style: von Bredow, C., von Bredow, Y. and Trenczek, T. (2021) The larval haematopoietic organs of Manduca sexta (Insecta, Lepidoptera): An insight into plasmatocyte development and larval haematopoiesis, Developmental & Comparative Immunology: Ontogeny, Phylogeny, Aging, 115, Article 103858. p. 103858. https://doi.org/10.1016/j.dci.2020.103858

APA Citation style: von Bredow, C., von Bredow, Y., & Trenczek, T. (2021). The larval haematopoietic organs of Manduca sexta (Insecta, Lepidoptera): An insight into plasmatocyte development and larval haematopoiesis. Developmental & Comparative Immunology: Ontogeny, Phylogeny, Aging. 115, Article 103858, 103858. https://doi.org/10.1016/j.dci.2020.103858