Journal article

Authors list: von Bülow, V; Gindner, S; Baier, A; Hehr, L; Buss, N; Russ, L; Wrobel, S; Wirth, V; Tabatabai, K; Quack, T; Haeberlein, S; Kadesch, P; Gerbig, S; Wiedemann, KR; Spengler, B; Mehl, A; Morlock, G; Schramm, G; Pons-Kühnemann, J; Falcone, FH; Wilson, RA; Bankov, K; Wild, P; Grevelding, CG; Roeb, E; Roderfeld, M

Publication year: 2023

Journal: JHEP Reports

Volume number: 5

Issue number: 2

ISSN: 2589-5559

Open access status: Green

DOI Link: https://doi.org/10.1016/j.jhepr.2022.100625

Publisher: Elsevier

Abstract: 
Background & Aims

Schistosomiasis is a parasitic infection which affects more than 200 million people globally. Schistosome eggs, but not the adult worms, are mainly responsible for schistosomiasis-specific morbidity in the liver. It is unclear if S. mansoni eggs consume host metabolites, and how this compromises the host parenchyma.

Methods

Metabolic reprogramming was analyzed by matrix-assisted laser desorption/ionization mass spectrometry imaging, liquid chromatography with high-resolution mass spectrometry, metabolite quantification, confocal laser scanning microscopy, live cell imaging, quantitative real-time PCR, western blotting, assessment of DNA damage, and immunohistology in hamster models and functional experiments in human cell lines. Major results were validated in human biopsies.

Results

The infection with S. mansoni provokes hepatic exhaustion of neutral lipids and glycogen. Furthermore, the distribution of distinct lipid species and the regulation of rate-limiting metabolic enzymes is disrupted in the liver of S. mansoni infected animals. Notably, eggs mobilize, incorporate, and store host lipids, while the associated metabolic reprogramming causes oxidative stress-induced DNA damage in hepatocytes. Administration of reactive oxygen species scavengers ameliorates these deleterious effects.

Conclusions

Our findings indicate that S. mansoni eggs completely reprogram lipid and carbohydrate metabolism via soluble factors, which results in oxidative stress-induced cell damage in the host parenchyma.

Impact and implications

The authors demonstrate that soluble egg products of the parasite S. mansoni induce hepatocellular reprogramming, causing metabolic exhaustion and a strong redox imbalance. Notably, eggs mobilize, incorporate, and store host lipids, while the metabolic reprogramming causes oxidative stress-induced DNA damage in hepatocytes, independent of the host's immune response. S. mansoni eggs take advantage of the host environment through metabolic reprogramming of hepatocytes and enterocytes. By inducing DNA damage, this neglected tropical disease might promote hepatocellular damage and thus influence international health efforts.

Harvard Citation style: von Bülow, V., Gindner, S., Baier, A., Hehr, L., Buss, N., Russ, L., et al. (2023) Metabolic reprogramming of hepatocytes by Schistosoma mansoni eggs, JHEP Reports, 5(2), Article 100625. https://doi.org/10.1016/j.jhepr.2022.100625

APA Citation style: von Bülow, V., Gindner, S., Baier, A., Hehr, L., Buss, N., Russ, L., Wrobel, S., Wirth, V., Tabatabai, K., Quack, T., Haeberlein, S., Kadesch, P., Gerbig, S., Wiedemann, K., Spengler, B., Mehl, A., Morlock, G., Schramm, G., Pons-Kühnemann, J., ...Roderfeld, M. (2023). Metabolic reprogramming of hepatocytes by Schistosoma mansoni eggs. JHEP Reports. 5(2), Article 100625. https://doi.org/10.1016/j.jhepr.2022.100625