Journalartikel

Autorenliste: Pitann, B; Bakhat, HF; Fatima, A; Hanstein, S; Schubert, S

Jahr der Veröffentlichung: 2021

Seiten: 1121-1130

Zeitschrift: Plant Physiology and Biochemistry

Bandnummer: 166

ISSN: 0981-9428

eISSN: 1873-2690

DOI Link: https://doi.org/10.1016/j.plaphy.2021.07.016

Verlag: Elsevier

Abstract: 
Silicon (Si)-mediated growth promotion of various grasses is well documented. In the present study, Si-induced changes in maize shoot growth and its underlying mechanisms were studied. Maize plants were grown with various concentrations of Si (0-3 mM) in the nutrient solution. Silicon nutrition improved plant expansion growth. Silicon-supplied maize plants (0.8 and 1.2 mM) showed higher plant height and leaf area compared to no-Si amended plants. It was assumed that Si-induced expansion growth was due to positive Si effects on plasma membrane (PM) H+-ATPase. In this context, western blot analysis revealed an increase in PM H+-ATPase abundance by 77% under Si nutrition. However, in vitro measurements of enzyme activities showed no significant effect on apoplast pH, proton pumping, passive H+ efflux and enzyme kinetics such as K-m, V-max, and activation energy. Further, these results were confirmed by in vivo ratiometric analysis of apoplastic pH, which showed non-significant changes upon Si supply. In contrast, 1 mM Si altered the relative transcripts of specific PM H+-ATPase isoforms. Silicon application resulted in a significant decrease of MHA3, and this decrease in transcription seems to be compensated by an increased concentration of H+-ATPase protein. From these results, it can be concluded that changes in cell wall composition and PM H+-ATPase may be responsible for Si-mediated growth improvement in maize.

Harvard-Zitierstil: Pitann, B., Bakhat, H., Fatima, A., Hanstein, S. and Schubert, S. (2021) Silicon-mediated growth promotion in maize (Zea mays L.) occurs via a mechanism that does not involve activation of the plasma membrane H+-ATPase, Plant Physiology and Biochemistry, 166, pp. 1121-1130. https://doi.org/10.1016/j.plaphy.2021.07.016

APA-Zitierstil: Pitann, B., Bakhat, H., Fatima, A., Hanstein, S., & Schubert, S. (2021). Silicon-mediated growth promotion in maize (Zea mays L.) occurs via a mechanism that does not involve activation of the plasma membrane H+-ATPase. Plant Physiology and Biochemistry. 166, 1121-1130. https://doi.org/10.1016/j.plaphy.2021.07.016