Journal article

Authors list: Töpfer, U; Bischoff, MC; Bartkuhn, M; Holz, A

Publication year: 2019

Journal: Scientific Reports

Volume number: 9

ISSN: 2045-2322

Open access status: Gold

DOI Link: https://doi.org/10.1038/s41598-019-52210-9

Publisher: Nature Research

Abstract: 
Transcriptional regulation of Laminin expression during embryogenesis is a key step required for proper ECM assembly. We show, that in Drosophila the Laminin B1 and Laminin B2 genes share expression patterns in mesodermal cells as well as in endodermal and ectodermal gut primordia, yolk and amnioserosa. In the absence of the GATA transcription factor Serpent, the spatial extend of Laminin reporter gene expression was strongly limited, indicating that Laminin expression in many tissues depends on Serpent activity. We demonstrate a direct binding of Serpent to the intronic enhancers of Laminin B1 and Laminin B2. In addition, ectopically expressed Serpent activated enhancer elements of Laminin B1 and Laminin B2. Our results reveal Serpent as an important regulator of Laminin expression across tissues.

Harvard Citation style: Töpfer, U., Bischoff, M., Bartkuhn, M. and Holz, A. (2019) Serpent/dGATAb regulates Laminin B1 and Laminin B2 expression during Drosophila embryogenesis, Scientific Reports, 9, Article 15910. https://doi.org/10.1038/s41598-019-52210-9

APA Citation style: Töpfer, U., Bischoff, M., Bartkuhn, M., & Holz, A. (2019). Serpent/dGATAb regulates Laminin B1 and Laminin B2 expression during Drosophila embryogenesis. Scientific Reports. 9, Article 15910. https://doi.org/10.1038/s41598-019-52210-9