Journalartikel

Autorenliste: Bergmaier, P; Weth, O; Dienstbach, S; Boettger, T; Galjart, N; Mernberger, M; Bartkuhn, M; Renkawitz, R

Jahr der Veröffentlichung: 2018

Seiten: 7097-7107

Zeitschrift: Nucleic Acids Research

Bandnummer: 46

Heftnummer: 14

ISSN: 0305-1048

eISSN: 1362-4962

Open Access Status: Gold

DOI Link: https://doi.org/10.1093/nar/gky483

Verlag: Oxford University Press

Abstract: 
The two paralogous zinc finger factors CTCF and CTCFL differ in expression such that CTCF is ubiquitously expressed, whereas CTCFL is found during spermatogenesis and in some cancer types in addition to other cell types. Both factors share the highly conserved DNA binding domain and are bound to DNA sequences with an identical consensus. In contrast, both factors differ substantially in the number of bound sites in the genome. Here, we addressed the molecular features for this binding specificity. In contrast to CTCF we found CTCFL highly enriched at 'open' chromatin marked by H3K27 acetylation, H3K4 di- and trimethylation, H3K79 dimethylation and H3K9 acetylation plus the histone variant H2A.Z. CTCFL is enriched at transcriptional start sites and regions bound by transcription factors. Consequently, genes deregulated by CTCFL are highly cell specific. In addition to a chromatin-driven choice of binding sites, we determined nucleotide positions critical for DNA binding by CTCFL, but not by CTCF.

Harvard-Zitierstil: Bergmaier, P., Weth, O., Dienstbach, S., Boettger, T., Galjart, N., Mernberger, M., et al. (2018) Choice of binding sites for CTCFL compared to CTCF is driven by chromatin and by sequence preference , Nucleic Acids Research, 46(14), pp. 7097-7107. https://doi.org/10.1093/nar/gky483

APA-Zitierstil: Bergmaier, P., Weth, O., Dienstbach, S., Boettger, T., Galjart, N., Mernberger, M., Bartkuhn, M., & Renkawitz, R. (2018). Choice of binding sites for CTCFL compared to CTCF is driven by chromatin and by sequence preference . Nucleic Acids Research. 46(14), 7097-7107. https://doi.org/10.1093/nar/gky483