Journal article

Authors list: Babylon, L; Schmitt, F; Franke, Y; Hubert, T; Eckert, GP

Publication year: 2022

Journal: International Journal of Molecular Sciences

Volume number: 23

Issue number: 15

eISSN: 1422-0067

Open access status: Gold

DOI Link: https://doi.org/10.3390/ijms23158670

Publisher: MDPI

Abstract: 
Increased amyloid beta (A beta) levels and mitochondrial dysfunction (MD) in the human brain characterize Alzheimer disease (AD). Folic acid, magnesium and vitamin B6 are essential micro-nutrients that may provide neuroprotection. Bioenergetic parameters and amyloid precursor protein (APP) processing products were investigated in vitro in human neuroblastoma SH-SY5Y-APP695 cells, expressing neuronal APP, and in vivo, in the invertebrate Caenorhabditis elegans (CL2006 & GMC101) expressing muscular APP. Model organisms were incubated with either folic acid and magnesium-orotate (ID63) or folic acid, magnesium-orotate and vitamin B6 (ID64) in different concentrations. ID63 and ID64 reduced A beta, soluble alpha APP (sAPP alpha), and lactate levels in SH-SY5Y-APP695 cells. The latter might be explained by enhanced expression of lactate dehydrogenase (LDHA). Micronutrient combinations had no effects on mitochondrial parameters in SH-SY5Y-APP695 cells. ID64 showed a significant life-prolonging effect in C. elegans CL2006. Incubation of GMC101 with ID63 significantly lowered A beta aggregation. Both combinations significantly reduced paralysis and thus improved the phenotype in GMC101. Thus, the combinations of the tested biofactors are effective in pre-clinical models of AD by interfering with A beta related pathways and glycolysis.

Harvard Citation style: Babylon, L., Schmitt, F., Franke, Y., Hubert, T. and Eckert, G. (2022) Effects of Combining Biofactors on Bioenergetic Parameters, Aβ Levels and Survival in Alzheimer Model Organisms, International Journal of Molecular Sciences, 23(15), Article 8670. https://doi.org/10.3390/ijms23158670

APA Citation style: Babylon, L., Schmitt, F., Franke, Y., Hubert, T., & Eckert, G. (2022). Effects of Combining Biofactors on Bioenergetic Parameters, Aβ Levels and Survival in Alzheimer Model Organisms. International Journal of Molecular Sciences. 23(15), Article 8670. https://doi.org/10.3390/ijms23158670