Journal article

Authors list: Baumanns, S; Schmitt, F; Spahn, C; Ringelmann, AE; Beis, DM; Eckert, GP; Wenzel, U

Publication year: 2024

Pages: 252-261

Journal: Nutritional Neuroscience: An International Journal on Nutrition, Diet and Nervous System

Volume number: 27

Issue number: 3

ISSN: 1028-415X

eISSN: 1476-8305

DOI Link: https://doi.org/10.1080/1028415X.2023.2180870

Publisher: Taylor and Francis Group

Abstract: 
Computer-based analysis of motility was used as a measure of amyloid-beta (A beta) proteotoxicity in the transgenic strain GMC101, expressing human A beta(1-42) in body wall muscle cells. A beta-aggregation was quantified to relate the effects of caprylic acid (CA) to the amount of the proteotoxic protein. Gene knockdowns were induced through RNA-interference (RNAi). Moreover, the estimation of adenosine triphosphate (ATP) levels, the mitochondrial membrane potential (MMP) and oxygen consumption served the evaluation of mitochondrial function. CA improved the motility of GMC101 nematodes and reduced A beta aggregation. Whereas RNAi for orthologues encoding key enzymes for alpha-lipoic acid and ketone bodies synthesis did not affect motility stimulation by CA, knockdown of orthologues involved in beta-oxidation of fatty acids diminished its effects. The efficient energy gain by application of CA was finally proven by the increase of ATP levels in association with increased oxygen consumption and MMP. In conclusion, CA attenuates A beta proteotoxicity by supplying energy via FAO. Since especially glucose oxidation is disturbed in Alzheimer's disease, CA could potentially serve as an alternative energy fuel.

Harvard Citation style: Baumanns, S., Schmitt, F., Spahn, C., Ringelmann, A., Beis, D., Eckert, G., et al. (2024) Caprylic acid attenuates amyloid-β proteotoxicity by supplying energy via β-oxidation in an Alzheimer’s disease model of the nematode Caenorhabditis elegans, Nutritional Neuroscience: An International Journal on Nutrition, Diet and Nervous System, 27(3), pp. 252-261. https://doi.org/10.1080/1028415X.2023.2180870

APA Citation style: Baumanns, S., Schmitt, F., Spahn, C., Ringelmann, A., Beis, D., Eckert, G., & Wenzel, U. (2024). Caprylic acid attenuates amyloid-β proteotoxicity by supplying energy via β-oxidation in an Alzheimer’s disease model of the nematode Caenorhabditis elegans. Nutritional Neuroscience: An International Journal on Nutrition, Diet and Nervous System. 27(3), 252-261. https://doi.org/10.1080/1028415X.2023.2180870