Journalartikel

Autorenliste: Wagner, E; Engelhardt, OG; Weber, F; Haller, O; Kochs, G

Jahr der Veröffentlichung: 2000

Seiten: 2849-2853

Zeitschrift: Journal of General Virology

Bandnummer: 81

Heftnummer: 12

ISSN: 0022-1317

DOI Link: https://doi.org/10.1099/0022-1317-81-12-2849

Verlag: Microbiology Society

Abstract: 
Thogoto virus (THOV) is the type species of tick-transmitted orthomyxoviruses. Here, we describe the generation of virus-like particles (VLP) of THOV from cloned cDNAs. To synthesize the six structural proteins of THOV in mammalian cells, we used T7-controlled expression plasmids and a recombinant vaccinia virus producing T7 RNA polymerase. A minireplicon encoding a reporter gene flanked by THOV promoter sequences was expressed by the cellular RNA polymerase I. The recombinant proteins were functional in encapsidation, amplification and transcription of the minireplicon RNA. Furthermore, the artificial nucleocapsids were packaged into THO-VLPs that transferred the minireplicon to indicator cells. This system should be helpful in generating recombinant THOV entirely from cloned cDNAs.

Harvard-Zitierstil: Wagner, E., Engelhardt, O., Weber, F., Haller, O. and Kochs, G. (2000) Formation of virus-like particles from cloned cDNAs of Thogoto virus, Journal of General Virology, 81(12), pp. 2849-2853. https://doi.org/10.1099/0022-1317-81-12-2849

APA-Zitierstil: Wagner, E., Engelhardt, O., Weber, F., Haller, O., & Kochs, G. (2000). Formation of virus-like particles from cloned cDNAs of Thogoto virus. Journal of General Virology. 81(12), 2849-2853. https://doi.org/10.1099/0022-1317-81-12-2849