Journal article

Authors list: Wagner, E; Engelhardt, OG; Weber, F; Haller, O; Kochs, G

Publication year: 2000

Pages: 2849-2853

Journal: Journal of General Virology

Volume number: 81

Issue number: 12

ISSN: 0022-1317

DOI Link: https://doi.org/10.1099/0022-1317-81-12-2849

Publisher: Microbiology Society

Abstract: 
Thogoto virus (THOV) is the type species of tick-transmitted orthomyxoviruses. Here, we describe the generation of virus-like particles (VLP) of THOV from cloned cDNAs. To synthesize the six structural proteins of THOV in mammalian cells, we used T7-controlled expression plasmids and a recombinant vaccinia virus producing T7 RNA polymerase. A minireplicon encoding a reporter gene flanked by THOV promoter sequences was expressed by the cellular RNA polymerase I. The recombinant proteins were functional in encapsidation, amplification and transcription of the minireplicon RNA. Furthermore, the artificial nucleocapsids were packaged into THO-VLPs that transferred the minireplicon to indicator cells. This system should be helpful in generating recombinant THOV entirely from cloned cDNAs.

Harvard Citation style: Wagner, E., Engelhardt, O., Weber, F., Haller, O. and Kochs, G. (2000) Formation of virus-like particles from cloned cDNAs of Thogoto virus, Journal of General Virology, 81(12), pp. 2849-2853. https://doi.org/10.1099/0022-1317-81-12-2849

APA Citation style: Wagner, E., Engelhardt, O., Weber, F., Haller, O., & Kochs, G. (2000). Formation of virus-like particles from cloned cDNAs of Thogoto virus. Journal of General Virology. 81(12), 2849-2853. https://doi.org/10.1099/0022-1317-81-12-2849