Journal article

Authors list: Kochs, G; Weber, F; Gruber, S; Delvendahl, A; Leitz, C; Haller, O

Publication year: 2000

Pages: 10785-10789

Journal: Journal of Virology

Volume number: 74

Issue number: 22

ISSN: 0022-538X

eISSN: 1098-5514

DOI Link: https://doi.org/10.1128/JVI.74.22.10785-10789.2000

Publisher: American Society for Microbiology

Abstract: 
Thogoto vints (THOV) is a tick-transmitted orthomyxovirus with a segmented, negative-stranded RNA genome. In this study, we investigated the coding strategy of RNA segment 6 and found that it contains 956 nucleotides and codes for the matrix (M) protein. The full-length cDNA contains a single, long reading frame that lacks a stop codon but has coding capacity for a putative 35-kDa protein. In contrast, the M protein of THOV has an apparent molecular mass of 29 kDa as assessed by polyacrylamide gel electrophoresis. Therefore, we investigated the possibility of posttranscriptional processing of segment 6 transcripts by reverse transcription-PCR and identified a spliced mRNA that contains a stop codon and is translated into the 29-kDa M protein. Interestingly, the nontemplated UGA stop codon is generated by the splicing event itself. Thus, the unusual M coding strategy of THOV resembles that of Influenza C virus.

Harvard Citation style: Kochs, G., Weber, F., Gruber, S., Delvendahl, A., Leitz, C. and Haller, O. (2000) Thogoto Virus Matrix Protein Is Encoded by a Spliced mRNA, Journal of Virology, 74(22), pp. 10785-10789. https://doi.org/10.1128/JVI.74.22.10785-10789.2000

APA Citation style: Kochs, G., Weber, F., Gruber, S., Delvendahl, A., Leitz, C., & Haller, O. (2000). Thogoto Virus Matrix Protein Is Encoded by a Spliced mRNA. Journal of Virology. 74(22), 10785-10789. https://doi.org/10.1128/JVI.74.22.10785-10789.2000