Journal article

Authors list: Jennings, S; Martínez-Sobrido, L; García-Sastre, A; Weber, F; Kochs, G

Publication year: 2005

Pages: 63-72

Journal: Virology

Volume number: 331

Issue number: 1

ISSN: 0042-6822

DOI Link: https://doi.org/10.1016/j.virol.2004.10.015

Publisher: Elsevier

Abstract: 

The Thogoto virus (THOV) is a member of the family Orthomyxoviridae. It prevents induction of alphabeta interferons (IFN) in cell culture and in vivo via the action of the viral ML protein. Phenotypically. the effect of THOV ML resembles that of the NSI protein of influenza A virius (FLUAV) in that it blocks the expression of IFN genes. IFN expression depends on IFN regulatory factor 3 (IRF3). Upon activation, IRF3 forms homodimers and accumulates in the nucleus where it binds the transcriptional coactivator CREB-binding protein (CBP). Here, we show that expression of ML blocked the transcriptional activity of IRF3 after stimulation by virus infection. Further biochemical analysis revealed that ML acts by blocking lRF3 dimerization and association with CBP. Surprisingly. however. ML did not interfere with the nuclear transport of IRF3. Thus. the action of ML differs strikingly front that of FLUAV NS1 that prevents IFN induction by retaining IRF3 in the cytoplasm.

Harvard Citation style: Jennings, S., Martínez-Sobrido, L., García-Sastre, A., Weber, F. and Kochs, G. (2005) Thogoto virus ML protein suppresses IRF3 function, Virology, 331(1), pp. 63-72. https://doi.org/10.1016/j.virol.2004.10.015

APA Citation style: Jennings, S., Martínez-Sobrido, L., García-Sastre, A., Weber, F., & Kochs, G. (2005). Thogoto virus ML protein suppresses IRF3 function. Virology. 331(1), 63-72. https://doi.org/10.1016/j.virol.2004.10.015