Journal article

Authors list: Spiegel, M; Schneider, K; Weber, F; Weidmann, M; Hufert, FT

Publication year: 2006

Pages: 1953-1960

Journal: Journal of General Virology

Volume number: 87

Issue number: 7

ISSN: 0022-1317

eISSN: 1465-2099

DOI Link: https://doi.org/10.1099/vir.0.81624-0

Publisher: Microbiology Society

Abstract: 
Severe acute respiratory syndrome (SARS) of humans is caused by a novel coronavirus of zoonotic origin termed SARS-associated coronavirus (SARS-CoV). The virus induces severe injury of lung tissue, as well as lymphopenia. and destruction of the architecture of lymphatic tissue by as-yet-unknown mechanisms. In this study, the interaction of SAIRS-CoV with dendritic cells (DCs), the key regulators of immune responses, was analysed. Monocyte-clerived DCs were infected with SARS-CoV and analysed for viability, surface-marker expression and alpha interferon (IFN-alpha) induction. SAIRS-CoV infection was monitored by quantitative RT-PCIR, immunofluorescence analysis and recovery experiments. SARS-CoV infected both immature and mature DCs, although replication efficiency was low. Immature DiCs were activated by SARS-CoV infection and by UV-inactivated SARS-CoV. Infected DCs were still viable on day 6 post-infection, but major histocompatibility complex class I upregulation was missing, indicating that DC function was impaired. Additionally, SARS-CoV infection induced a delayed activation of IFN-alpha expression. Therefore, it is concluded that SARS-CoV has the ability to circumvent both the innate and the adaptive immune systems.

Harvard Citation style: Spiegel, M., Schneider, K., Weber, F., Weidmann, M. and Hufert, F. (2006) Interaction of severe acute respiratory syndrome-associated coronavirus with dendritic cells, Journal of General Virology, 87(7), pp. 1953-1960. https://doi.org/10.1099/vir.0.81624-0

APA Citation style: Spiegel, M., Schneider, K., Weber, F., Weidmann, M., & Hufert, F. (2006). Interaction of severe acute respiratory syndrome-associated coronavirus with dendritic cells. Journal of General Virology. 87(7), 1953-1960. https://doi.org/10.1099/vir.0.81624-0