Tula and Puumala hantavirus NSs ORFs are functional and the products inhibit activation of the interferon-beta promoter - Research portal of Justus Liebig University Giessen:

Journal article

Tula and Puumala hantavirus NSs ORFs are functional and the products inhibit activation of the interferon-beta promoter

Authors list: Jääskeläinen, KM; Kaukinen, P; Minskaya, ES; Plyusnina, A; Vapalahti, O; Elliott, RM; Weber, F; Vaheri, A; Plyusnin, A

Publication year: 2007

Pages: 1527-1536

Journal: Journal of Medical Virology

Volume number: 79

Issue number: 10

ISSN: 0146-6615

eISSN: 1096-9071

DOI Link: https://doi.org/10.1002/jmv.20948

Publisher: Wiley

Abstract:
The S RNA genome segment of hantaviruses carried by Arvicolinae and Sigmodontinae rodents encodes the nucleocapsid (N) protein and has an overlapping (+1) open reading frame (ORF) for a putative nonstructural protein (NSs). The aim of this study was to determine whether the ORF is functional. A protein corresponding to the predicted size of Tula virus (TULV) NSs was detected using coupled in vitro transcription and translation from a cloned S segment cDNA, and a protein corresponding to the predicted size of Puumala virus (PUUV) NSs was detected in infected cells by Western blotting with an anti-peptide serum. The activities of the interferon beta (IFN-beta) promoter, and nuclear factor kappa B (NF-kappa beta)- and interferon regulatory factor-3 (IRF-3) responsive promoters, were inhibited in COS-7 cells transiently expressing TULV or PUUV NSs. Also IFN-beta mRNA levels in IFN-competent MRC5 cells either infected with TULV or transiently expressing NSs were decreased. These data demonstrate that Tula and Puumala hantaviruses have a functional NSs ORE The findings may explain why the NSs ORF has been preserved in the genome of most hantaviruses during their long evolution and why hantavirus-infected cells secrete relatively low levels of lFNs.

Authors/Editors

- Uni.-Prof. Dr. Friedemann Johannes Weber

Citation Styles

Harvard Citation style: Jääskeläinen, K., Kaukinen, P., Minskaya, E., Plyusnina, A., Vapalahti, O., Elliott, R., et al. (2007) Tula and Puumala hantavirus NSs ORFs are functional and the products inhibit activation of the interferon-beta promoter, Journal of Medical Virology, 79(10), pp. 1527-1536. https://doi.org/10.1002/jmv.20948

APA Citation style: Jääskeläinen, K., Kaukinen, P., Minskaya, E., Plyusnina, A., Vapalahti, O., Elliott, R., Weber, F., Vaheri, A., & Plyusnin, A. (2007). Tula and Puumala hantavirus NSs ORFs are functional and the products inhibit activation of the interferon-beta promoter. Journal of Medical Virology. 79(10), 1527-1536. https://doi.org/10.1002/jmv.20948